A0A6S6QLX9 · PLE2_RHOPP

Function

function

Acetyltransferase; part of the gene cluster that mediates the biosynthesis of pleuromutilin, a tricyclic diterpene showing antibacterial properties (PubMed:28924980).
The geranylgeranyl diphosphate (GGPP) synthase ple4 catalyzes the first step in pleuromutilin biosynthesis (PubMed:28924980).
GGPP is then substrate of the premutilin synthase (PS) ple3 to yield premutilin (PubMed:28924980).
Premutilin synthase is a bifunctional enzyme composed of the fusion of a class II diterpene cyclase (DTC) and a class I diterpene synthase (DTS), with the corresponding domains and active sites containing characteristic aspartate-rich motifs (By similarity).
GGPP is first converted to mutildienyl-diphosphate (MPP) at the class II DTC site (By similarity).
MPP is subsequently further cyclized at the class I DTS site, followed by a 1,5-hydride shift and addition of water prior to terminating deprotonation, to yield premutilin (By similarity).
The cytochrome P450 monooxygenases ple5 and ple6 hydroxylate premutilin at C-11 and C-3, respectively, producing 11-hydroxypremutilin and 3-hydroxypremutilin (PubMed:28924980).
The combination of the actions of both ple5 and ple6 leads to the production of 3,11-dihydroxypremutilin (PubMed:28924980).
The short chain dehydrogenase ple7 further converts 3,11-dihydroxypremutilin into mutilin (PubMed:28924980).
The acetyltransferase ple2 then acetylates mutilin to produce 14-O-acetylmutilin (PubMed:28924980).
Finally, the cytochrome P450 monooxygenase ple1 catalyzes hydroxylation on the alpha position of the acetyl side chain of 14-O-acetylmutilin to yield pleuromutilin (PubMed:28924980).

Pathway

Secondary metabolite biosynthesis; terpenoid biosynthesis.

GO annotations

AspectTerm
Cellular Componentmembrane
Molecular FunctionO-acyltransferase activity
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Acetyltransferase ple2
  • EC number
  • Alternative names
    • Pleuromutilin biosynthesis cluster protein 2

Gene names

    • Name
      ple2

Organism names

Accessions

  • Primary accession
    A0A6S6QLX9

Subcellular Location

Membrane
; Multi-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane29-49Helical
Transmembrane56-76Helical
Transmembrane176-196Helical
Transmembrane258-278Helical

Keywords

PTM/Processing

Features

Showing features for signal, chain.

TypeIDPosition(s)Description
Signal1-27
ChainPRO_502829675128-377Acetyltransferase ple2

Family & Domains

Sequence similarities

Belongs to the wax synthase family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    377
  • Mass (Da)
    43,171
  • Last updated
    2020-12-02 v1
  • Checksum
    06AD422A1E07C484
MKPFSPELLVLSFILLVLSCAIRPAKGRWILWVIIVALNTYLTMTTTGDSTLDYDIANNLFVITLTATDYILLTDVQRELQFRNQKGVEQASLLERIKWATWLVQSRRGVGWNWEPKIFVHRFSPKTSRLSFLLQQLVTGARHYLICDLVSLYSRSPVAFAEPLASRPLIWRCADIAAWLLFTTNQVSILLTALSLMQVLSGYSEPQDWVPVFGRWRDAYTVRRFWGRSWHQLVRRCLSSPGKYLSTKVLGLKPGSNPALYVQLYAAFFLSGVLHAIGDFKVHEDWYKAGTMEFFCVQAVIIQMEDGVLWVGRKLGIKETWYWRALGHLWTVAWFVYSCPNWLGATISGRGKASMALESSLVLGLYRGEWHPPRVAQ

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LC314149
EMBL· GenBank· DDBJ
BCI98770.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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