A0A6S6M5J5 · A0A6S6M5J5_9BACT
- ProteintRNA-dihydrouridine synthase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids321 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines.
Catalytic activity
- a 5,6-dihydrouridine in tRNA + NAD+ = a uridine in tRNA + NADH + H+
- a 5,6-dihydrouridine in tRNA + NADP+ = a uridine in tRNA + NADPH + H+
Cofactor
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 20-22 | FMN (UniProtKB | ChEBI) | ||||
Sequence: PMA | ||||||
Binding site | 74 | FMN (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Active site | 103 | Proton donor | ||||
Sequence: C | ||||||
Binding site | 142 | FMN (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 172 | FMN (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 227-228 | FMN (UniProtKB | ChEBI) | ||||
Sequence: AR |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | RNA binding | |
Molecular Function | tRNA dihydrouridine synthase activity |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-dihydrouridine synthase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Thermodesulfobacteriota > Desulfuromonadia > Geobacterales > Geobacteraceae > Citrifermentans
Accessions
- Primary accessionA0A6S6M5J5
Proteomes
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 18-311 | DUS-like FMN-binding | ||||
Sequence: LAPMAGITNLPMRIISREGGASFAFTEMVSVNGLTREGRKSFDLLQSSAEDRPIGMQLFGDDPEMLAEAARLVEEYGELIDINMGCPVRKVVGTGAGSALMKDPLKVGRIVRSVRKATKLPLTIKIRTGWVCGDDTFMEVGRIAQEEGCDAVTLHPRSRAQMFEGKADWSRIGELKSALSIPVIGSGDLFSAADAVGMLTQTGCDAVMVARGAMGNPWIFREALSLLAGEEPVPPTVKERLAVSRRHLELFTEFAGGRVALMEMRKHLSWYSKGLPGAAQFRAAVNRIESTAEL |
Sequence similarities
Belongs to the dus family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length321
- Mass (Da)34,817
- Last updated2020-12-02 v1
- Checksum22C8D6519723EFA3
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP023213 EMBL· GenBank· DDBJ | BCG46665.1 EMBL· GenBank· DDBJ | Genomic DNA |