A0A6Q8PF33 · A0A6Q8PF33_HUMAN
- ProteinAlanine--tRNA ligase
- GeneAARS1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids961 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.
Catalytic activity
- tRNA(Ala) + L-alanine + ATP = L-alanyl-tRNA(Ala) + AMP + diphosphate
Cofactor
Note: Binds 1 zinc ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 598 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 602 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 716 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 720 | Zn2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | alanine-tRNA ligase activity | |
Molecular Function | ATP binding | |
Molecular Function | tRNA binding | |
Molecular Function | zinc ion binding | |
Biological Process | alanyl-tRNA aminoacylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAlanine--tRNA ligase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionA0A6Q8PF33
Proteomes
Organism-specific databases
Subcellular Location
Disease & Variants
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 1,148 variants from UniProt as well as other sources including ClinVar and dbSNP.
Genetic variation databases
PTM/Processing
Features
Showing features for modified residue, modified residue (large scale data).
Type | ID | Position(s) | Source | Description | ||
---|---|---|---|---|---|---|
Modified residue | 1 | UniProt | N-acetylmethionine | |||
Modified residue (large scale data) | 399 | PRIDE | Phosphoserine | |||
Modified residue (large scale data) | 403 | PRIDE | Phosphoserine | |||
Modified residue (large scale data) | 484 | PRIDE | Phosphoserine | |||
Modified residue (large scale data) | 660 | PRIDE | Phosphotyrosine | |||
Post-translational modification
ISGylated.
Keywords
- PTM
Proteomic databases
Expression
Gene expression databases
Interaction
Subunit
Monomer. Interacts with ANKRD16; the interaction is direct.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 5-759 | Alanyl-transfer RNA synthetases family profile | |||
Domain
Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.
Sequence similarities
Belongs to the class-II aminoacyl-tRNA synthetase family. Alax-L subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length961
- Mass (Da)106,063
- Last updated2020-12-02 v1
- ChecksumC74957FC42BCF19F
Computationally mapped potential isoform sequences
There are 17 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
P49588 | SYAC_HUMAN | AARS1 | 968 | ||
A0A6Q8PFK3 | A0A6Q8PFK3_HUMAN | AARS1 | 772 | ||
A0A6Q8PFY2 | A0A6Q8PFY2_HUMAN | AARS1 | 977 | ||
A0A6Q8PG28 | A0A6Q8PG28_HUMAN | AARS1 | 327 | ||
A0A6Q8PG62 | A0A6Q8PG62_HUMAN | AARS1 | 278 | ||
A0A6Q8PGN5 | A0A6Q8PGN5_HUMAN | AARS1 | 984 | ||
A0A6Q8PGE8 | A0A6Q8PGE8_HUMAN | AARS1 | 959 | ||
A0A6Q8PGI8 | A0A6Q8PGI8_HUMAN | AARS1 | 275 | ||
A0A6Q8PGB5 | A0A6Q8PGB5_HUMAN | AARS1 | 925 | ||
A0A6Q8PGR9 | A0A6Q8PGR9_HUMAN | AARS1 | 940 | ||
A0A6Q8PH44 | A0A6Q8PH44_HUMAN | AARS1 | 734 | ||
A0A6Q8PF77 | A0A6Q8PF77_HUMAN | AARS1 | 521 | ||
A0A6Q8PHN5 | A0A6Q8PHN5_HUMAN | AARS1 | 48 | ||
A0A6Q8PHP3 | A0A6Q8PHP3_HUMAN | AARS1 | 535 | ||
A0A6Q8PHP7 | A0A6Q8PHP7_HUMAN | AARS1 | 560 | ||
A0A6Q8PHJ2 | A0A6Q8PHJ2_HUMAN | AARS1 | 789 | ||
H3BPK7 | H3BPK7_HUMAN | AARS1 | 999 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC009060 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AC012184 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AC093491 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |