A0A6P6LLB9 · A0A6P6LLB9_CARAU

Function

function

Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends.

Catalytic activity

  • Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.
    EC:3.1.11.2 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Probably binds two magnesium or manganese ions per subunit.
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site, site.

130820406080100120140160180200220240260280300
TypeIDPosition(s)Description
Binding site59Mg2+ 1 (UniProtKB | ChEBI)
Binding site87Mg2+ 1 (UniProtKB | ChEBI)
Active site162
Active site201Proton donor/acceptor
Binding site201Mg2+ 1 (UniProtKB | ChEBI)
Binding site203Mg2+ 1 (UniProtKB | ChEBI)
Site203Transition state stabilizer
Site273Important for catalytic activity
Binding site298Mg2+ 1 (UniProtKB | ChEBI)
Active site299Proton acceptor
Binding site299Mg2+ 1 (UniProtKB | ChEBI)
Site299Interaction with DNA substrate

GO annotations

AspectTerm
Cellular Componentmitochondrion
Cellular Componentnucleus
Molecular FunctionDNA binding
Molecular FunctionDNA-(apurinic or apyrimidinic site) endonuclease activity
Molecular Functiondouble-stranded DNA 3'-5' DNA exonuclease activity
Molecular Functionendonuclease activity
Molecular Functionlyase activity
Molecular Functionmetal ion binding
Molecular Functionphosphoric diester hydrolase activity
Biological Processbase-excision repair

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    exodeoxyribonuclease III
  • EC number
  • Alternative names
    • APEX nuclease
    • Apurinic-apyrimidinic endonuclease 1

Gene names

    • Name
      LOC113059822

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Ostariophysi > Cypriniformes > Cyprinidae > Cyprininae > Carassius

Accessions

  • Primary accession
    A0A6P6LLB9

Proteomes

Subcellular Location

Nucleus
Cytoplasm
Mitochondrion

Keywords

Family & Domains

Features

Showing features for compositional bias, region, domain.

TypeIDPosition(s)Description
Compositional bias1-48Basic and acidic residues
Region1-50Disordered
Domain57-299Endonuclease/exonuclease/phosphatase

Sequence similarities

Belongs to the DNA repair enzymes AP/ExoA family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    308
  • Mass (Da)
    34,903
  • Last updated
    2020-12-02 v1
  • Checksum
    BFCA45A72091A57E
MPKRAKKNEEAVEGETGNEPAKKEKKGKEPEAPILYEDPPDKTSSKDGRASNMKITSWNVDGLRAWVKKKGLDWVRKEDPDVLCLQETKCAEKALPSEITDMPEYPHKYWAGSEEKEGYSGVAMLCKTEPLRVTYGIGKEEHGKEGRVITAEFPSFFLVTAYVPNASRGLVRLDYRKTWDVDFRAYLSVLDQRKPLVLCGDLNVAHQEIDLKNPKGNRKNAGFTPEEREGFTKLLEAGFTDSFRELYPEQANAYTFWTYMMNARAKNVGWRLDYFLLSSALLPGLCDSKIRNTAMGSDHCPITLYLAV

Computationally mapped potential isoform sequences

There are 3 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A6P6LLA1A0A6P6LLA1_CARAULOC113059822341
A0A6P6LLA5A0A6P6LLA5_CARAULOC113059822339
A0A6P6LI19A0A6P6LI19_CARAULOC113059822310

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias1-48Basic and acidic residues

Keywords

Sequence databases

Similar Proteins

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