A0A6N7CWG9 · A0A6N7CWG9_9NOCA

  • Protein
    Lysine--tRNA ligase
  • Gene
    lysX
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Catalyzes the production of L-lysyl-tRNA(Lys)transfer and the transfer of a lysyl group from L-lysyl-tRNA(Lys) to membrane-bound phosphatidylglycerol (PG), which produces lysylphosphatidylglycerol (LPG), one of the components of the bacterial membrane with a positive net charge. LPG synthesis contributes to the resistance to cationic antimicrobial peptides (CAMPs) and likely protects M.tuberculosis against the CAMPs produced by competiting microorganisms (bacteriocins). In fact, the modification of anionic phosphatidylglycerol with positively charged L-lysine results in repulsion of the peptides.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 3 Mg2+ ions per subunit.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site1024Mg2+ 1 (UniProtKB | ChEBI)
Binding site1031Mg2+ 2 (UniProtKB | ChEBI)
Binding site1031Mg2+ 1 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentplasma membrane
Molecular FunctionATP binding
Molecular Functionlysine-tRNA ligase activity
Molecular Functionmagnesium ion binding
Molecular Functionnucleic acid binding
Molecular Functionphosphatidylglycerol lysyltransferase activity
Biological Processlipid metabolic process
Biological Processlysyl-tRNA aminoacylation
Biological Processresponse to antibiotic

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Lysine--tRNA ligase
  • EC number
  • Alternative names
    • Lysyl-tRNA synthetase
      (LysRS
      )

Gene names

    • Name
      lysX
    • Synonyms
      lysS
    • ORF names
      MLGJGCBP_00786

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • T7
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Nocardiaceae > Rhodococcus

Accessions

  • Primary accession
    A0A6N7CWG9

Proteomes

Subcellular Location

Cell membrane
; Multi-pass membrane protein
Cytoplasm
Membrane
; Multi-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane37-60Helical
Transmembrane80-95Helical
Transmembrane102-122Helical
Transmembrane128-145Helical
Transmembrane157-180Helical
Transmembrane218-239Helical

Keywords

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-21Disordered
Domain792-1111Aminoacyl-transfer RNA synthetases class-II family profile

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.
In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.
In the N-terminal section; belongs to the LPG synthetase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,113
  • Mass (Da)
    122,294
  • Last updated
    2020-10-07 v1
  • Checksum
    F58C83C0F077E6E0
MSASTETHHASEADLTAPRPRPALGSKNGRLHQVPHIAGLILGVFSVLVFLWSISPVLRYYVHVPREYIDTYYFDAPDTSLSWALVVALLAAALASRKRIAWWLLTIYLTLILITNVIVSITDRNVNAMVAAVVQVVLIGILIAARPEFYTRVRRGAGWKALGVLIVGLAIGTLVGWGLVELFPGTLPQGERFLWALNRVTALAAADNEQFSGRPHGFVNTLLGLFGALALLAAVITLFRAQRSHNALTGNDESALRGLLLQYGADDSLGYFATRRDKAVVFAPSGKAAITYRVELGVCLASGDPIGDPEAWPHAIEAWQALASQYGWATAVMGASETGATAYNKAGLTVLQLGDEAILRTREFNLSGRDMRQVRQAVTRVRRQGITVRIRRHRDVPPDEMAEAIRLADSWRDTETERGFSMALGRLGDRLDGDCLLVEAVAEDGEIDGILSLVPWGPTGVSLDLMRRRPTSPNGVVELMVSELATNSDQFGITKVSLNFAVFRSVFEEGSRIGAGPILRIWRSILVFFSRWWQLEALYRSNVKYQPEWVPRFLCFEDNRELLRVGFASAVAEGFVTLPRFGRSGTHDAIEHTGHHAAVPAALVAAEGLHSDGSAPGEGLAPTTTGPKRPEQVRVRLDKLAGLAEQGIDPYPVALPPSHTVTEAVESPEGTTVRIAGRLLRIRDYGGVVFAVVRDWSGDIQILVDEARVGTDRIRAFAAEFDLGDLVEVAGVIGYSRRGALSLLANEWRMTGKCLHPLPDKWKGLSDPETRVRQRYVDLAINSDARRLLEARSAVVKSLRDSLGGRGFLEVETPILQQVHGGANAAPFLTHINAYNLDLYLRIAPELYLKRLCVAGMEKVFEIGRVFRNEGVDFKHNPEFTILEAYEAHSDYEKMMVLCRELIQTAAVAAYGREIIMRPGPDGRLVEVDISGEWPVKTMHQAVAEKLGVDVSPETPLAELQRLCDEHEIPYQSAWDAGAVAQEMYEHLVEDYTEFPTFYTNFPTSMSPLTRPHPTIPGVAAKWDLVAWGIELGTAYSELTDPVDQRNRLTEQSMLAAGGDEEAMELDEDFLQALEHAMPPTGGLGMGVDRVVMLITGGSIRETLAFPLAKPRQ

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
WXYH01000024
EMBL· GenBank· DDBJ
KAF0966059.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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