A0A6M2RI12 · A0A6M2RI12_9POTV

  • Protein
    Genome polyprotein
  • Gene
    ORF1
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Has helicase activity. It may be involved in replication.
Involved in aphid transmission, cell-to-cell and systemis movement, encapsidation of the viral RNA and in the regulation of viral RNA amplification.
Mediates the cap-independent, EIF4E-dependent translation of viral genomic RNAs. Binds to the cap-binding site of host EIF4E and thus interferes with the host EIF4E-dependent mRNA export and translation. VPg-RNA directly binds EIF4E and is a template for transcription. Also forms trimeric complexes with EIF4E-EIF4G, which are templates for translation.

Catalytic activity

  • Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.
    EC:3.4.22.45 (UniProtKB | ENZYME | Rhea)
  • Hydrolyzes glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or Gly) for the enzyme from tobacco etch virus. The natural substrate is the viral polyprotein, but other proteins and oligopeptides containing the appropriate consensus sequence are also cleaved.
    EC:3.4.22.44 (UniProtKB | ENZYME | Rhea)

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site998For helper component proteinase activity
Active site1071For helper component proteinase activity

GO annotations

AspectTerm
Cellular Componenthelical viral capsid
Cellular Componenthost cell cytoplasmic vesicle
Cellular Componenthost cell nucleus
Molecular FunctionATP binding
Molecular Functioncysteine-type endopeptidase activity
Molecular Functionhelicase activity
Molecular Functionhydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
Molecular FunctionRNA binding
Molecular FunctionRNA-dependent RNA polymerase activity
Molecular Functionserine-type peptidase activity
Molecular Functionstructural molecule activity
Biological ProcessDNA-templated transcription
Biological Processproteolysis
Biological Processsymbiont-mediated suppression of host innate immune response
Biological Processviral RNA genome replication
Biological Processviral translational frameshifting
Biological Processvirus-mediated perturbation of host defense response

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Genome polyprotein

Gene names

    • Name
      ORF1

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • GS
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Pisuviricota > Stelpaviricetes > Patatavirales > Potyviridae > Potyvirus > Potyvirus cebatatae

Accessions

  • Primary accession
    A0A6M2RI12

Subcellular Location

Keywords

PTM/Processing

Keywords

Family & Domains

Features

Showing features for compositional bias, region, domain.

Type
IDPosition(s)Description
Compositional bias200-216Basic and acidic residues
Region200-223Disordered
Domain511-654Peptidase S30
Domain990-1112Peptidase C6
Domain1588-1740Helicase ATP-binding
Domain1759-1918Helicase C-terminal
Domain2405-2623Peptidase C4
Domain2889-3013RdRp catalytic
Region3164-3243Disordered
Compositional bias3203-3215Basic and acidic residues
Compositional bias3225-3234Basic and acidic residues

Sequence similarities

Belongs to the potyviridae genome polyprotein family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    3,481
  • Mass (Da)
    393,396
  • Last updated
    2020-10-07 v1
  • MD5 Checksum
    759F7A1ADFCC21E4C760E657BF5F801A
MASATVSVKPAGKRKLTWKECCNKWGKAAMEQQQATYPRRKVELSRNQLAANIFAFVPETEWHKYYFGNRGGPSPQLEVLSNAIQYGEVFKYKFDFTFCPECDEFLEGHKCEECGTIFRKTDDNLADNMNVVARELGGYDAYYASTWSEFEQAKLELEEVAPTAGQLRRQARAAEKLLDKRSTRREKEYVDSLWEAVEEAETKETGEASDVSEKAAELSGISSSATADEMFPPLVGNKDTEVITTTSDVIVSECPNIQFGQINEFTPEEGKDEKVVESLVEEAENVTVPAVMPIETGFFFGTIPAIIPLPVVVPQQGDMGVLQPAFMQVGTIPVNGIARADTHLEIKETAVKKTEPTKEDTDVGAQKSTSLPAHLYPWAPRSKNSGATRHQMVRKWVKKTQEAEEMKQKAVWKKLDEQLAVRNEAKKDLKVKWRWGLYRLVKKTRKDNQRQRQKKRMEKEQQLLLAMPPQILTGISIAGGPVASLQETPTVSGKILSTPSMKRKKILKSPTLSSDKIQELIQATLKIACKQGLQVEIINKKTVKGAYRQHGGTNHLFLHLKHMEGWRKPVDLHIHQEDIELVTMAARVGAWNKKFRTSQLCRGTSGLVLNPDKLTGPRGHAPKGLFVVRGALAGVVYDARMRLGRSILPFIEQFSSTMERFLRGFDEKFKQLRQVDSDHVCESTYDAEQAGAVAAIIHHMVCPMNRTTCKHCSNKIEDLSKDEWCDYVRNFANKNKLPFQLEFKDFKHLPLIIDFITDSLVQTNKDVRAFNEIQTLIGNRTDAPFTTVCEVNKVLVKGGRAKSAEFAKASEALLELARYLKNRTENIKKGSLQSFRNKISQKAAMNLALMCDNQLDKNGNLIWGERGYHSKRFFSNYFEEINPEEGYSKYIVRSNPNGTRKLAIGKLIVSTNFSVFREQMKGEPIHKQKLDNHCTSTLDGNFIYPCCCVTYDDGQPMESEFKLPTKNHLVIGNSGDPKYVDMPPEISKKMYIAKEGYCYINIFLAMLVNVNEAEAKDFTKQVRDVLMEKLGKWPTMFDVATACAFMSVFYPETRNAELPRILIDHTTKTMHVIDSFGSLSTGYHILKANTVSQLIKFANSSLDSEMKHYLVGGLTALPANEERCVRTVIKGVYRPKILYEILSEDPYMLMLSVISPRVILALFNSGSLDRAMDTWITKDQEVAVIVGTLSELARKVSTSRVLEQQLQIIESQSHTLLFDDSTTRRRTPSFALSQRVIRSLSERRETNRVLHEQGHTVSAYASSHELMEKIWDTLLRQEYAELTLLEKCSLIMRSSRYMRCIQGTYLRQSLDDFRGRVDASYTALHTKSVALSNTCKEGFVSRVADVHARCVRTIVQTSLSAIRFAVPDILKFVNLLLVVNLILQIAHVARNMVQKHQQAQIDLNAYLFDQEIDKVNVIYDSLCKKVGGEPTMDEFLAHIEYINPTLLGTAKWLCYTADVEVEHQGKSRKEIQYERIIAFISLLLMVVDSERSDCVYKILSKLRGLMGCIDGGVYHQSLDDITSILEEKNLTIDFELQGDEQLSNVMTDATFDEWWRKQLETNRTITHYRTEGTFIEFTRSNAVSVANNIATLPTNDILVRGAVGSGKSTGLPFYLSRKGRVLLLEPTRPLAENVHRQLGGDPFMVQTTLRMRGLTVFGSHPINIMTTGFAFHYYANNPEQLREYEFIIFDECHVNDAQAMAFRCLLKEHEFSGKIIKASATPPGREVDFATQHPVQIKVEESLSFKAFVEAQGKGSNADVVAVADNILVYVASYNEVDELSRLLVEANHKVTKVDGRTMKVGSVEIKTTGTSSKKHFIVATNIIENGVTLDIEAVVDFGTKVTAYLDVDLRMIRTSKGPISYGERIQRLGRVGRNKAGTALRIGYTEKGLVDIPQTVATEAAFLCFAYGLPVMTPNVSTSLLGSCTVKQARTMLQFELTPFYMVNMVRFDGSMHHAVHQLLKRYKLRDAETTLNKLAIPNRGITGWMSAADYRKVGQRLDIEDQIRIPFYHQSMPEKLHSDIWEAIQRYKHEAGFGRVSCINSCKIAYTLQTDVYAIPRTIKILDALIADEMRKKEHYKTITGRTVSSSSFTLNSIATLWRNRYAQDYTSENIAVLSSARAQLLEFENLSMDASYNEMSKGILETYVRENGATTCVLHQTKSDLAKHLQLKGIWNKSVITQDLMVLAGVFVGGIWMVFSGAKESFEQTVKHQGKEKRQRQKLKFRQARDRKLGFEVHADDGTIEHYFGSAYTAKGKQKGKVTGMGAKNRKFINMYGFDPTEYSFVRFVDPLTGLVFDDSPYTDIQLVQEKIGDARHKAILEDKLSRERISFNPGISAYYINEITNAALKVDLTPHNPLLACERHSTIAGHPDHEGELRQTGSVVKIDIRDVPENPEEASLVGHESKSLFRGLRDYNPIASVICHLVNEADGRTSDSYGIGYGGLIITNRHLFKRNNGTLTIRSRHGEFVIKNTTQLGMKPCADRDILIIKMPKDIPPFPQRLQFRIPKENERICLVGSNFQDKSITSTVSETSVTCHVPNSHFWKHWIDTKDGHCGLPLVSTVDGAILGVHSLSNLTNTQNFFAAVPANFEIDYLKTPEATEWIKKWSYNPSEICWGTLELKTGQPVAPFKVTKLITDLDSMQVYAQARSDRWVLDRLYGNLKAVGQCPAQLVTKHVVKGKCMLFDLYLQQDCDVRSFFKPLMGAYGKSRLNKEAYNKDLFKYATPINAGDVCHKTFEQAELAVVDMLKANGFHKCNYITDPEEIFKALNMKAAVGAMYSGKKKDYFDGMSDEDIENLLFHSCKRLYMGQKGLWNGSLKAELRPMAKVELNKTRTFTAAPLDTLLGGKTCVDDFNNMFYNNHLKCPWTVGITKFYQGWDRLLTSLPEDWIYCDADGSQFDSSLSPYLINAVLNIRREFMEEWDIGDQMLRNLYTEIVYTPILTPDGTIVKKFKGNNSGQPSTVVDNTLMVVLAVYYTLLKLGVSELEISERCVFFANGDDLLIAIRPDTAHLLDNFSECFAELGLNYDFSSRTTKREELWFMSHCGIKRDGIYIPKLEPERIVSILEWDRSHEPIHRLEAICAAMVEAWGYDELLHQIRKFYAWVLGQAPYSELARTGKAPYIAETALRSLYTGVQPSADDLSEYTRVLNEMYDDSLLQDNDLTVYHQSGNPPEFKDAGANPPAPKPKGPYTAPEITEITEPEDPKQAALREARQKQPAVTPESYGRDTGEKPMRSVSPQRVKDKDVNVGTTGTFLVPRVKLHTSKMRQPRVNGISVVNLQHLATYEPEQHNIGNTRSTQEQFQAWYEGVKGDYGVDDAGMAILLNGLMVWCIENGTSPNINGVWTMMDGDEQVTYPIKPLLDHAVPTFRQIMTHFSDVAEAYIEMRNRTKAYMPRYGLQRNLTDLSLARYAFDFYELNSTTPARAKEAHMQMKAAALKNAQNRLFGLDGNISTQEEDTERHTTTDVTRNIHNLLGMRGVQ

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias200-216Basic and acidic residues
Compositional bias3203-3215Basic and acidic residues
Compositional bias3225-3234Basic and acidic residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MH388498
EMBL· GenBank· DDBJ
QCG75819.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

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