A0A6L7IGI0 · A0A6L7IGI0_9FLAO

Function

function

Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

methylcob(III)alamin (UniProtKB | Rhea| CHEBI:28115 )

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetH route): step 1/1.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site355methylcob(III)alamin (UniProtKB | ChEBI)
Binding site418-422methylcob(III)alamin (UniProtKB | ChEBI)
Binding site421Co (UniProtKB | ChEBI) of methylcob(III)alamin (UniProtKB | ChEBI); axial binding residue
Binding site466methylcob(III)alamin (UniProtKB | ChEBI)
Binding site470methylcob(III)alamin (UniProtKB | ChEBI)
Binding site522methylcob(III)alamin (UniProtKB | ChEBI)
Binding site608S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site796S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site851-852S-adenosyl-L-methionine (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functioncobalamin binding
Molecular Functionmethionine synthase activity
Molecular Functionzinc ion binding
Biological Processhomocysteine metabolic process
Biological Processmethylation
Biological Processtetrahydrofolate metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Methionine synthase
  • EC number
  • Alternative names
    • 5-methyltetrahydrofolate--homocysteine methyltransferase

Gene names

    • Name
      metH
    • ORF names
      GSF70_07625

Organism names

Accessions

  • Primary accession
    A0A6L7IGI0

Proteomes

Subcellular Location

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain20-281Pterin-binding
Domain311-405B12-binding N-terminal
Domain408-543B12-binding
Domain558-888AdoMet activation

Domain

Modular enzyme with four functionally distinct domains. The isolated Hcy-binding domain catalyzes methyl transfer from free methylcobalamin to homocysteine. The Hcy-binding domain in association with the pterin-binding domain catalyzes the methylation of cob(I)alamin by methyltetrahydrofolate and the methylation of homocysteine. The B12-binding domain binds the cofactor. The AdoMet activation domain binds S-adenosyl-L-methionine. Under aerobic conditions cob(I)alamin can be converted to inactive cob(II)alamin. Reductive methylation by S-adenosyl-L-methionine and flavodoxin regenerates methylcobalamin.

Sequence similarities

Belongs to the vitamin-B12 dependent methionine synthase family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    888
  • Mass (Da)
    100,195
  • Last updated
    2020-10-07 v1
  • MD5 Checksum
    7EE8D7443B3ED006DB5351D330403EB9
MKYLRLSGLEPLIITPESNFINVGERTNVAGSKKFLRLIKEEKFGEALDIARHQVEGGAQILDVNFDDGLIDGKASMIKFLNLIASEPDIARIPLMIDSSKWEILEAGLQVAQGKCVVNSISLKGGEEEFIKQAKAIKRYGAAVIVMAFDEVGQADTYERRIEISKRSYDILVNRLNFPAEDIIFDLNIFPVATGMDEHRRNAIDFIEATRWVRQNLPYASVSGGVSNVSFSFRGNDTVREAMHSVFLYHAIQAGMNMGIVNPAMLEVYDEINKELLELVEDVILDKREDATERLLDYSEKHKSVKKEKVEELEWRTKPLQERITHSLVKGIDRFIEEDVEEARLQAEKPLHVIEINLMTGMGVVGDLFGSGKMFLPQVVKSARVMKKAVAYLQPFIEAEKDGTKPANGKILMATVKGDVHDIGKNIVSVVLGCNNYEIVDLGVMVPAEKIIQAAIDHQVDVIGLSGLITPSLDEMVYIAQELERQNLNFPLLIGGATTSKAHTAVKIDLKYKNAVVHVNDASRAVNVVSSLLGDRNKEYVDDLKTEYSEFREKFLNRQVEKDYVSIEVARQNKFKIDWENEEIFTPNQLGITVVENQDLRELLPFIDWSPFFRSWDLHGKYPQILEDEVVGAQAKELFKDAQVILKRILDEKLLTAKAIFGIFKANSNETDDILIFDENNEQKTKFITLRQQAQRSKGKDYLALSDFIAPESSGKTDYMGAFCVTTGFGTDELAAEYEKANDDYNAIMVKALADRFAEAYAEFLHKKVRTEYWGYAYQENLTNEELIAEKYKGIRPAPGYPACPDHLEKHAIWDLLKVEENIGVYLTESLAMFPTASVSGYYFGSPHAKYFGLGKIAEDQLKDYSKRKGISLQEARKWLNPNLADGI

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
WUWH01000015
EMBL· GenBank· DDBJ
MXS71083.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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