A0A6L7GPH4 · A0A6L7GPH4_9ACTN

  • Protein
    Isoleucine--tRNA ligase
  • Gene
    ileS
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site641ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionaminoacyl-tRNA editing activity
Molecular FunctionATP binding
Molecular Functionisoleucine-tRNA ligase activity
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological Processisoleucyl-tRNA aminoacylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Isoleucine--tRNA ligase
  • EC number
  • Alternative names
    • Isoleucyl-tRNA synthetase
      (IleRS
      )

Gene names

    • Name
      ileS
    • ORF names
      GIY30_07160

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • HNM0687
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Gordoniaceae > Gordonia

Accessions

  • Primary accession
    A0A6L7GPH4

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain, motif.

TypeIDPosition(s)Description
Domain43-672Aminoacyl-tRNA synthetase class Ia
Motif73-83'HIGH' region
Motif638-642'KMSKS' region
Domain720-861Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,074
  • Mass (Da)
    119,394
  • Last updated
    2020-10-07 v1
  • Checksum
    6CC3AD0ABC872368
MADTAQPDAANEQSAEGRVYPVVDMTAGGGRSAPSFPDVEQHVLAYWADDDTFAASIENRADAEEFVFYDGPPFANGLPHYGHLLTGYVKDLVPRYQTMRGKRVERRFGWDTHGLPAELEAERQLGITDKSQIESMGMEKFNDYCRDSVLRYTGEWRDYVTRQARWVDFDNDYKTLDLDFMESVMWAFKRLHDKGLVYQGYRVLPYSWYEQTPLSNQESKLDDAYRMRQDPAVTVRMPLVGTDAHPELDGVNALIWTTTPWTLPSNLAIAVNPDVEYVHVRAADGQEYLLAKALLAGYAKEIVEPEILGSYGGADLEGMSYLPPFDFFVGHPNAHRVLLGDYVTTDSGTGVVHLAPAFGEEDMDLAMANGIEVVQPLDPGGRFTAQVPPYEGQMVFDANPAIIKDLKAIGKILRHDTIEHSYPHSWRSGKPLIYMAVPSWFVAVTPIKERMLELNKQITWSPEHIRDGQFGKWLEGARDWNISRNRFWGAPIPVWISDDPEHPRTDVYGSLDELERDFGVRPDNLHRPYIDELTRPNPDDPTGNSTMRRVPEVFDCWFESGSMPFAQVHYPFENADWFDGNSDSGDVPHNPGDFIVEYNGQSRGWFYNLHVLATALFDRPAFKSVIAHGIVLGDDGQKMSKSKRNYPDVNEVFDRDGSDAMRWFLMASPVLRGGNLVVTERGIREGVRQALLPLWNAYSFLQLYAERPATWRTDSTHVLDRYILAKLAVTRDTMTEALDTFDIAGACEAFREFVESLTNWYVRRSRARFWAGQDDDPDAFDTLYTVLEVAARLAAPLLPLATEAIWRGLTGGRSVHLTDWPAADDLPADTALVAAMDEVQEVCSVASSVRKANKLRVRLPLPGLTVASPTAEQLTPYRDLIADEMNVKSVSLATDADAYGRYEIAVNARAAGPRLGKDVQAAIKAVKSGDWTVGAGADGTEVVIAGGIELRDGEYTRRLVAVEPDSTAELPGGRGLVVLDTAVTPELEAEGWAKDRIRELQDARRNLALDVSDRIVVRLVVPAQRVAWATAHAGLIAGEVLAVTFDVVEADGDQPAGAIELGDGVTADVAKVSA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
WMBR01000001
EMBL· GenBank· DDBJ
MXP21131.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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