A0A6L5EEI6 · A0A6L5EEI6_9ENTR
- ProteinQuinate/shikimate dehydrogenase
- GeneydiB
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids264 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
The actual biological function of YdiB remains unclear, nor is it known whether 3-dehydroshikimate or quinate represents the natural substrate. Catalyzes the reversible NAD-dependent reduction of both 3-dehydroshikimate (DHSA) and 3-dehydroquinate to yield shikimate (SA) and quinate, respectively. It can use both NAD or NADP for catalysis, however it has higher catalytic efficiency with NAD.
Catalytic activity
- L-quinate + NAD+ = 3-dehydroquinate + NADH + H+
Pathway
Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 47 | substrate | ||||
Sequence: K | ||||||
Binding site | 83 | substrate | ||||
Sequence: D | ||||||
Binding site | 108-111 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: AGGA | ||||||
Binding site | 181 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 208-211 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: CVYN | ||||||
Binding site | 231 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: G |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | NADP binding | |
Molecular Function | quinate 3-dehydrogenase (NAD+) activity | |
Molecular Function | quinate 3-dehydrogenase (NADP+) activity | |
Molecular Function | shikimate 3-dehydrogenase (NAD+) activity | |
Molecular Function | shikimate 3-dehydrogenase (NADP+) activity | |
Biological Process | amino acid biosynthetic process | |
Biological Process | aromatic amino acid family biosynthetic process | |
Biological Process | chorismate biosynthetic process | |
Biological Process | shikimate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameQuinate/shikimate dehydrogenase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Citrobacter
Accessions
- Primary accessionA0A6L5EEI6
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 1-70 | Shikimate dehydrogenase substrate binding N-terminal | ||||
Sequence: MQNKALEKAGLPFTYMAFEVDNSTFAGAIEGLKALKMRGTGVSMPNKQLACEFVDELTPAAKLVGAINTI | ||||||
Domain | 231-260 | SDH C-terminal | ||||
Sequence: GYGMLLWQGAEQFKLWTGKDFPLEYVKQVM |
Sequence similarities
Belongs to the shikimate dehydrogenase family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length264
- Mass (Da)28,589
- Last updated2020-10-07 v1
- Checksum9D5B8012A4C6CD48