A0A6J7U7I4 · A0A6J7U7I4_9ZZZZ
- ProteinLysylphosphatidylglycerol biosynthesis bifunctional protein LysX
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1092 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the production of L-lysyl-tRNA(Lys)transfer and the transfer of a lysyl group from L-lysyl-tRNA(Lys) to membrane-bound phosphatidylglycerol (PG), which produces lysylphosphatidylglycerol (LPG), one of the components of the bacterial membrane with a positive net charge. LPG synthesis contributes to the resistance to cationic antimicrobial peptides (CAMPs) and likely protects M.tuberculosis against the CAMPs produced by competiting microorganisms (bacteriocins). In fact, the modification of anionic phosphatidylglycerol with positively charged L-lysine results in repulsion of the peptides.
Catalytic activity
- L-lysyl-tRNA(Lys) + a 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) = a 1,2-diacyl-sn-glycero-3-phospho-1'-(3'-O-L-lysyl)-sn-glycerol + tRNA(Lys)
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | plasma membrane | |
Molecular Function | ATP binding | |
Molecular Function | lysine-tRNA ligase activity | |
Molecular Function | phosphatidylglycerol lysyltransferase activity | |
Molecular Function | tRNA binding | |
Biological Process | lipid metabolic process | |
Biological Process | lysyl-tRNA aminoacylation | |
Biological Process | response to antibiotic |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameLysylphosphatidylglycerol biosynthesis bifunctional protein LysX
- EC number
Gene names
Organism names
- Organism
- Taxonomic lineageunclassified sequences > metagenomes > ecological metagenomes
Accessions
- Primary accessionA0A6J7U7I4
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 531-553 | Helical | |||
Transmembrane | 565-584 | Helical | |||
Transmembrane | 596-612 | Helical | |||
Transmembrane | 618-634 | Helical | |||
Transmembrane | 654-672 | Helical | |||
Transmembrane | 709-730 | Helical | |||
Keywords
- Cellular component
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 168-486 | Aminoacyl-transfer RNA synthetases class-II family profile | |||
Sequence similarities
In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.
In the N-terminal section; belongs to the LPG synthetase family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,092
- Mass (Da)119,626
- Last updated2020-10-07 v1
- MD5 ChecksumCB4CE65AFBBCCE14DB2E2AFF69870ACE
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CAFBQW010000013 EMBL· GenBank· DDBJ | CAB5061362.1 EMBL· GenBank· DDBJ | Genomic DNA |