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A0A6J5M4Y2 · A0A6J5M4Y2_9CAUD

Function

function

The terminase large subunit acts as an ATP driven molecular motor necessary for viral DNA translocation into empty capsids and as an endonuclease that cuts the viral genome at a unique and precise dsDNA sequence to initiate and to end a packaging reaction. The terminase lies at a unique vertex of the procapsid and is composed of two subunits, a small terminase subunit involved in viral DNA recognition (packaging sequence), and a large terminase subunit possessing endonucleolytic and ATPase activities. Both terminase subunits heterooligomerize and are docked on the portal protein to form the packaging machine. The terminase large subunit exhibits endonuclease activity and cleaves the viral genome concatemer. Once the DNA is packaged, the terminase detaches from the portal and gets replaced by the tail to finish maturation of the virion.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site.

155150100150200250300350400450500550
TypeIDPosition(s)Description
Binding site344Mg2+ 1 (UniProtKB | ChEBI); catalytic; for nuclease activity
Binding site344Mg2+ 2 (UniProtKB | ChEBI); catalytic; for nuclease activity
Binding site400Mg2+ 2 (UniProtKB | ChEBI); catalytic; for nuclease activity
Binding site498Mg2+ 1 (UniProtKB | ChEBI); catalytic; for nuclease activity

GO annotations

AspectTerm
Cellular Componentviral terminase, large subunit
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular Functionendonuclease activity
Molecular Functionmetal ion binding
Biological Processchromosome organization
Biological Processviral DNA genome packaging

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Terminase, large subunit
  • Alternative names
    • DNA-packaging protein

Including 2 domains:

  • Recommended name
    ATPase
  • EC number
  • Recommended name
    Endonuclease
  • EC number

Gene names

    • ORF names
      UFOVP403_35

Organism names

Accessions

  • Primary accession
    A0A6J5M4Y2

Subcellular Location

Interaction

Subunit

Homopentamer. Interacts with the terminase small subunit; the active complex is probably heterooligomeric. Interacts with the portal protein.

Family & Domains

Features

Showing features for motif, region, domain.

Type
IDPosition(s)Description
Motif49-56Walker A motif
Region324-409Nuclease activity
Domain343-450Terminase large subunit ribonuclease H-like

Domain

The ATPase region is in the N-terminus, whereas the nuclease region is in the central part. The C-terminus is involved in prohead binding.

Sequence similarities

Belongs to the Teseptimavirus large terminase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    551
  • Mass (Da)
    61,849
  • Last updated
    2020-10-07 v1
  • MD5 Checksum
    CA8938108CCB25B447E49C32BA491ADB
MNEIDPRLKDFRNFLYIVWHHLGLPKPTKVQYDIADYIQNGPRRSVVMAFRGVGKSWITSAYVCHQLLLDPSKNILVVSASKGRADDFSTFTLRLIQEIPILNHLLPKESQRFSKVAFDVGPAPPQHAPSVTSKGITSQLTGSRADIVIADDVEVPNNSATQNMREKLAESIKEFEAVLKPNGKVIFLGTPQTESSIYNLLPDRGYNICIWPVRAPSEKDRVNYGDRLARFVSSLKPSELVEPDRFDGKELLERELSYGRSGFSLQYMLDTSLSDQDRYPLKINDLLVMDLNTELAPQKLIWASGTDQAMQDLHCVGFNGDRYHRPMALVGEWMPYNGSVMAIDPSGRGSDETAYAVVKMLNGNLFVTDAGGIAGGYGQAVLDKLGAIAKAQKVNQILVEENFGQGMFAELLKPVLTKVHPCAVELTRHSIQKEKRIIDTLEPVMNQHRLVFDAGVIKRDYTSTKDLPTEKALHYQLMYQMSRVTRSKGALAHDDRLDALAMAVAYWTEQMAQDADRKIAMNRSKAMDRELEKFMNAVVGRKPQGDTWMKV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LR796375
EMBL· GenBank· DDBJ
CAB4140543.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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