A0A6J4K4W3 · A0A6J4K4W3_9BACT
- ProteinEpoxyqueuosine reductase
- GenequeG
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids392 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the conversion of epoxyqueuosine (oQ) to queuosine (Q), which is a hypermodified base found in the wobble positions of tRNA(Asp), tRNA(Asn), tRNA(His) and tRNA(Tyr).
Catalytic activity
- epoxyqueuosine34 in tRNA + AH2 = queuosine34 in tRNA + A + H2O
Cofactor
Protein has several cofactor binding sites:
Note: Binds 2 [4Fe-4S] clusters per monomer.
Pathway
tRNA modification; tRNA-queuosine biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 64 | cob(II)alamin (UniProtKB | ChEBI) | |||
Active site | 137 | Proton donor | |||
Binding site | 137 | cob(II)alamin (UniProtKB | ChEBI) | |||
Binding site | 158 | cob(II)alamin (UniProtKB | ChEBI) | |||
Binding site | 161 | cob(II)alamin (UniProtKB | ChEBI) | |||
Binding site | 172 | cob(II)alamin (UniProtKB | ChEBI) | |||
Binding site | 192 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 195 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 198 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 202 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI) | |||
Binding site | 223 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI) | |||
Binding site | 225 | cob(II)alamin (UniProtKB | ChEBI) | |||
Binding site | 250 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI) | |||
Binding site | 250-251 | cob(II)alamin (UniProtKB | ChEBI) | |||
Binding site | 253 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI) | |||
Binding site | 257 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | cobalamin binding | |
Molecular Function | epoxyqueuosine reductase activity | |
Molecular Function | metal ion binding | |
Biological Process | queuosine biosynthetic process | |
Biological Process | tRNA modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameEpoxyqueuosine reductase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Gemmatimonadota > environmental samples
Accessions
- Primary accessionA0A6J4K4W3
Subcellular Location
Interaction
Subunit
Monomer.
Structure
Sequence
- Sequence statusComplete
- Length392
- Mass (Da)43,012
- Last updated2020-10-07 v1
- Checksum5FBD121E3D33E4ED
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CADCTW010000002 EMBL· GenBank· DDBJ | CAA9295747.1 EMBL· GenBank· DDBJ | Genomic DNA |