A0A6I8RB27 · A0A6I8RB27_XENTR
- ProteinCytosolic non-specific dipeptidase
- Genecndp2
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids466 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the peptide bond hydrolysis in dipeptides, displaying a non-redundant activity toward threonyl dipeptides. Mediates threonyl dipeptide catabolism in a tissue-specific way. Has high dipeptidase activity toward cysteinylglycine, an intermediate metabolite in glutathione metabolism. Metabolizes N-lactoyl-amino acids, both through hydrolysis to form lactic acid and amino acids, as well as through their formation by reverse proteolysis. Plays a role in the regulation of cell cycle arrest and apoptosis.
Catalytic activity
- (S)-lactate + L-phenylalanine = N-[(S)-lactoyl]-L-phenylalanine + H2OThis reaction proceeds in the forward and the backward directions.
- L-cysteinylglycine + H2O = L-cysteine + glycineThis reaction proceeds in the forward direction.
- L-seryl-L-threonine + H2O = L-threonine + L-serineThis reaction proceeds in the forward direction.
- L-threonyl-L-serine + H2O = L-threonine + L-serineThis reaction proceeds in the forward direction.
- L-threonyl-L-threonine + H2O = 2 L-threonineThis reaction proceeds in the forward direction.
Cofactor
Note: Binds 2 manganese ions per subunit.
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 91 | Mn2+ 2 (UniProtKB | ChEBI) | |||
Active site | 93 | ||||
Binding site | 124 | Mn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 124 | Mn2+ 1 (UniProtKB | ChEBI) | |||
Active site | 158 | Proton acceptor | |||
Binding site | 159 | Mn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 187 | Mn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 187 | substrate; ligand shared between homodimeric partners; in other chain | |||
Binding site | 220 | substrate; ligand shared between homodimeric partners | |||
Site | 220 | Important for catalytic activity | |||
Binding site | 322 | substrate; ligand shared between homodimeric partners | |||
Binding site | 335 | substrate; ligand shared between homodimeric partners; in other chain | |||
Binding site | 409 | substrate; ligand shared between homodimeric partners; in other chain | |||
Binding site | 437 | Mn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 437 | substrate; ligand shared between homodimeric partners; in other chain | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | carboxypeptidase activity | |
Molecular Function | metal ion binding | |
Molecular Function | metallodipeptidase activity |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCytosolic non-specific dipeptidase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Amphibia > Batrachia > Anura > Pipoidea > Pipidae > Xenopodinae > Xenopus > Silurana
Accessions
- Primary accessionA0A6I8RB27
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for signal, chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Signal | 1-25 | ||||
Chain | PRO_5027754270 | 26-466 | Cytosolic non-specific dipeptidase | ||
Keywords
- PTM
Expression
Gene expression databases
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length466
- Mass (Da)51,598
- Last updated2020-12-02 v1
- Checksum351D40380A77632E