A0A6I8P4R8 · A0A6I8P4R8_ORNAN
- ProteinInosine-5'-monophosphate dehydrogenase
- GeneIMPDH1
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids565 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.
Catalytic activity
- IMP + NAD+ + H2O = XMP + NADH + H+
Cofactor
Activity regulation
Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.
Pathway
Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 325-327 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: DSS | ||||||
Binding site | 375-377 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: GMG | ||||||
Binding site | 377 | K+ (UniProtKB | ChEBI); ligand shared between two tetrameric partners; in other chain | ||||
Sequence: G | ||||||
Binding site | 379 | K+ (UniProtKB | ChEBI); ligand shared between two tetrameric partners; in other chain | ||||
Sequence: G | ||||||
Binding site | 380 | IMP (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Active site | 382 | Thioimidate intermediate | ||||
Sequence: C | ||||||
Binding site | 382 | K+ (UniProtKB | ChEBI); ligand shared between two tetrameric partners; in other chain | ||||
Sequence: C | ||||||
Binding site | 415-417 | IMP (UniProtKB | ChEBI) | ||||
Sequence: DGG | ||||||
Binding site | 438-439 | IMP (UniProtKB | ChEBI) | ||||
Sequence: GS | ||||||
Binding site | 462-466 | IMP (UniProtKB | ChEBI) | ||||
Sequence: YRGMG | ||||||
Active site | 480 | Proton acceptor | ||||
Sequence: R | ||||||
Binding site | 492 | IMP (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 547 | K+ (UniProtKB | ChEBI); ligand shared between two tetrameric partners | ||||
Sequence: S |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | IMP dehydrogenase activity | |
Molecular Function | metal ion binding | |
Molecular Function | nucleotide binding | |
Biological Process | GMP biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameInosine-5'-monophosphate dehydrogenase
- EC number
- Short namesIMP dehydrogenase ; IMPD ; IMPDH
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Monotremata > Ornithorhynchidae > Ornithorhynchus
Accessions
- Primary accessionA0A6I8P4R8
Proteomes
Expression
Gene expression databases
Interaction
Subunit
Homotetramer.
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-37 | Disordered | ||||
Sequence: MEGQGRGPAGGAGAAHPGPAPEPGAPQPPAHRTAGQR | ||||||
Domain | 165-224 | CBS | ||||
Sequence: FITDPVVLSPSHTVGDVLEAKARHGFSGIPITETGTMGSKLVGIVTSRDIDFLAEKDHAT | ||||||
Domain | 230-288 | CBS | ||||
Sequence: MTRRTELVVAPAGVTLKEANEILQRSKKGKLPIVNDSDELVAIIARTDLKKNRDYPLAS |
Sequence similarities
Belongs to the IMPDH/GMPR family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length565
- Mass (Da)60,361
- Last updated2020-08-12 v1
- ChecksumD74DAD8DC81350CA
Computationally mapped potential isoform sequences
There are 4 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
F6PLN7 | F6PLN7_ORNAN | IMPDH1 | 571 | ||
A0A6I8PQP6 | A0A6I8PQP6_ORNAN | IMPDH1 | 532 | ||
A0A6I8P9U5 | A0A6I8P9U5_ORNAN | IMPDH1 | 489 | ||
F6PLM9 | F6PLM9_ORNAN | IMPDH1 | 482 |
Keywords
- Technical term