A0A6I3QT96 · A0A6I3QT96_9FIRM
- Protein3'-phosphate/5'-hydroxy nucleic acid ligase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids415 (go to sequence)
- Protein existencePredicted
- Annotation score2/5
Function
Catalytic activity
- a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP = a ribonucleotidyl-ribonucleotide-RNA + diphosphate + GMP
Cofactor
Note: Binds 2 manganese ions per subunit.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 87 | Mn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 159-163 | GMP (UniProtKB | ChEBI) | ||||
Sequence: NHFIE | ||||||
Binding site | 160 | Mn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 177 | Mn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 317-320 | GMP (UniProtKB | ChEBI) | ||||
Sequence: PINM | ||||||
Binding site | 324 | GMP (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Active site | 341 | GMP-histidine intermediate | ||||
Sequence: H | ||||||
Binding site | 341-344 | GMP (UniProtKB | ChEBI) | ||||
Sequence: HGAG |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | GTP binding | |
Molecular Function | metal ion binding | |
Molecular Function | RNA ligase (GTP) activity | |
Biological Process | RNA processing | |
Biological Process | RNA repair |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name3'-phosphate/5'-hydroxy nucleic acid ligase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillota > Clostridia > Eubacteriales > Oscillospiraceae > Ruthenibacterium
Accessions
- Primary accessionA0A6I3QT96
Proteomes
Structure
Sequence
- Sequence statusComplete
- Length415
- Mass (Da)45,775
- Last updated2020-08-12 v1
- ChecksumE2B83135F422B38D