A0A6I2F4E2 · A0A6I2F4E2_9MICO

  • Protein
    Glyceraldehyde-3-phosphate dehydrogenase
  • Gene
    gap
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.

Features

Showing features for binding site, active site, site.

Type
IDPosition(s)Description
Binding site12-13NAD+ (UniProtKB | ChEBI)
Binding site35NAD+ (UniProtKB | ChEBI)
Binding site79NAD+ (UniProtKB | ChEBI)
Binding site121NAD+ (UniProtKB | ChEBI)
Binding site152-154D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI)
Active site153Nucleophile
Site180Activates thiol group during catalysis
Binding site183D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI)
Binding site211-212D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI)
Binding site234D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI)
Binding site315NAD+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionglyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
Molecular FunctionNAD binding
Molecular FunctionNADP binding
Biological Processglucose metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Glyceraldehyde-3-phosphate dehydrogenase
  • EC number

Gene names

    • Name
      gap
    • ORF names
      GE115_06165

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • CFH 90414
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Micrococcales > Microbacteriaceae > Agromyces

Accessions

  • Primary accession
    A0A6I2F4E2

Proteomes

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain3-153Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding

Sequence similarities

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    333
  • Mass (Da)
    35,088
  • Last updated
    2020-08-12 v1
  • Checksum
    5E307A2DC6C2BB92
MTTRIAINGFGRIGRNVLRAIVERDADFEVVAVNDLTEPAALARLLKYDTAGGRFGHPVEVDGDTLVVAGRRVKVLAERDPAKLPWGDLDVELVLESTGRFTDAEAAKAHLAAGAKKVLVSAPSKGADVTLAYGVNTEAYDAAAHTIVSNASCTTNALAPLAKVLDDLAGIEHGFMTTVHAYTQDQNLQDAPHSDPRRARAAAENIVPSSTGAAKAIGLVLPGLDGKLDGDAMRVPIPVGSIVELNTTVAREVTRDEVLAAYRAAAEGPLAGVLEYSEEPLVSSDIVGNPHSSIFDAELTRVGGKHVKVVAWYDNEWGFSNRVIDSLGLLAKG

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
WJIF01000002
EMBL· GenBank· DDBJ
MRG59459.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp