A0A6H2BUD5 · A0A6H2BUD5_DOLFA

Function

function

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site14[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site50[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site84[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site156[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site160[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site163[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionmetal ion binding
Molecular Functionmethylthiotransferase activity
Biological Processnegative regulation of cyclin-dependent protein serine/threonine kinase activity
Biological ProcesstRNA modification

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
  • EC number
  • Alternative names
    • (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
    • tRNA-i(6)A37 methylthiotransferase

Gene names

    • Name
      miaB
    • ORF names
      HGD76_02060

Organism names

Accessions

  • Primary accession
    A0A6H2BUD5

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain5-121MTTase N-terminal
Domain142-379Radical SAM core
Domain382-446TRAM

Sequence similarities

Belongs to the methylthiotransferase family. MiaB subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    453
  • Mass (Da)
    51,324
  • Last updated
    2020-08-12 v1
  • Checksum
    3A7A6830F27EA127
MTTKRNYHIITFGCQMNKADSERMAGILEDMGFEWSEDPNQADLILYNTCTIRDNAEQKVYSYLGRQAKRKQEEPDLTLIVAGCVAQQEGAALLRRVPELDLVMGPQHANRLKDLLESVFAGNQVVATEEVHIFEDITQPRRDSQVTAWVNIIYGCNERCTYCVVPNVRGVEQSRTPEAVRSEIEQLARQGYKEITLLGQNIDAYGRDLPGSTPEGRHLHTLTDLLYYVHDVPGIERIRFATSHPRYFTERLIKACADLPKVCEHFHIPFQSGDNELLKAMSRGYTHEKYRRIIDTIRGYMPDASISADAIVGFPGETETQFENTLKLVEDIGFDLVNTAAYSPRPGTPAALWDNQLSEEVKSDRLQRLNHLVNIKAAERSQRYFGRIEEVLVEAQNTKDTNQVMGRTGGNRLTFFTGDINELKGQIVKVKITEVRAFSLTGEVVEVRQPVTV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP051206
EMBL· GenBank· DDBJ
QJB43195.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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