A0A6H1XEC6 · A0A6H1XEC6_9ROSA
- ProteinDNA-directed RNA polymerase subunit beta'
- GenerpoC1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids683 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity
- a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 Mg2+ ion per subunit.
Note: Binds 1 Zn2+ ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 69 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 71 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 87 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 90 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 490 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 492 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 494 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Cellular Component | DNA-directed RNA polymerase complex | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed 5'-3' RNA polymerase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | zinc ion binding | |
Biological Process | DNA-templated transcription |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerase subunit beta'
- EC number
- Alternative names
Gene names
Encoded on
- Chloroplast
Organism names
- Organism
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Rosales > Rosaceae > Amygdaloideae > Amygdaleae > Prunus
Accessions
- Primary accessionA0A6H1XEC6
Subcellular Location
Interaction
Subunit
In plastids the minimal PEP RNA polymerase catalytic core is composed of four subunits: alpha, beta, beta', and beta''. When a (nuclear-encoded) sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 263-544 | RNA polymerase N-terminal | ||||
Sequence: EWMVLCLLPVLPPELRPIIQIDGGKLMSSDINELYRRVIYRNNTLIDLLTTSRSTPGELVMCQEKLVQEAVDTLLDNGIRGQPMRDGHNKVYKSFSDVIEGKEGRFRETLLGKRVDYSGRSVIVVGPSLSLHRCGLPREIAIELFQTFVIRGLIRQHFASNIGVAKSKIREKEPVVWEILHEVMQGHPVLLNRAPTLHRLGIQAFQPILVEGHAICLHPLVCKGFNADFDGDQMAVHVPLSLEAQAEARLLMFSHMNLLSPAIGDPISVPTQDMLIGLYVLT |
Sequence similarities
Belongs to the RNA polymerase beta' chain family. RpoC1 subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length683
- Mass (Da)78,831
- Last updated2020-08-12 v1
- Checksum192CE8694D665EDB
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
MH700952 EMBL· GenBank· DDBJ | QJA13973.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
MW406457 EMBL· GenBank· DDBJ | QWK45284.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
MW406458 EMBL· GenBank· DDBJ | QWK45369.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
MW406459 EMBL· GenBank· DDBJ | QWK45454.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
MW406460 EMBL· GenBank· DDBJ | QWK45539.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
MW406461 EMBL· GenBank· DDBJ | QWK45624.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
MW406462 EMBL· GenBank· DDBJ | QWK45709.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
MW406465 EMBL· GenBank· DDBJ | QWK45964.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
MW406466 EMBL· GenBank· DDBJ | QWK46049.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
MW406467 EMBL· GenBank· DDBJ | QWK46134.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
MW406468 EMBL· GenBank· DDBJ | QWK46219.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
MW406469 EMBL· GenBank· DDBJ | QWK46304.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
MW406472 EMBL· GenBank· DDBJ | QWK46559.1 EMBL· GenBank· DDBJ | Genomic DNA |