A0A6H0IZI6 · A0A6H0IZI6_9GAMM

  • Protein
    ATP-dependent 6-phosphofructokinase
  • Gene
    pfkA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Activity regulation

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site23ATP (UniProtKB | ChEBI)
Binding site33-37ADP (UniProtKB | ChEBI); allosteric activator; ligand shared between dimeric partners
Binding site84-85ATP (UniProtKB | ChEBI)
Binding site114-117ATP (UniProtKB | ChEBI)
Binding site115Mg2+ (UniProtKB | ChEBI); catalytic
Binding site138-140substrate; ligand shared between dimeric partners; in other chain
Active site140Proton acceptor
Binding site167ADP (UniProtKB | ChEBI); allosteric activator; ligand shared between dimeric partners; in other chain
Binding site175substrate; ligand shared between dimeric partners
Binding site182-184substrate; ligand shared between dimeric partners; in other chain
Binding site198-200ADP (UniProtKB | ChEBI); allosteric activator; ligand shared between dimeric partners; in other chain
Binding site224ADP (UniProtKB | ChEBI); allosteric activator; ligand shared between dimeric partners; in other chain
Binding site226-228ADP (UniProtKB | ChEBI); allosteric activator; ligand shared between dimeric partners; in other chain
Binding site235substrate; ligand shared between dimeric partners; in other chain
Binding site257substrate; ligand shared between dimeric partners
Binding site263-266substrate; ligand shared between dimeric partners; in other chain

GO annotations

AspectTerm
Cellular Component6-phosphofructokinase complex
Molecular Function6-phosphofructokinase activity
Molecular FunctionAMP binding
Molecular FunctionATP binding
Molecular Functionfructose-6-phosphate binding
Molecular Functionidentical protein binding
Molecular Functionmetal ion binding
Molecular Functionmonosaccharide binding
Biological Processcanonical glycolysis
Biological Processfructose 1,6-bisphosphate metabolic process
Biological Processfructose 6-phosphate metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    ATP-dependent 6-phosphofructokinase
  • EC number
  • Short names
    ATP-PFK
    ; Phosphofructokinase
  • Alternative names
    • Phosphohexokinase

Gene names

    • Name
      pfkA
    • ORF names
      HC341_17385

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • 2447
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Chromatiales > Ectothiorhodospiraceae > Aquisalimonas

Accessions

  • Primary accession
    A0A6H0IZI6

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homotetramer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain15-288Phosphofructokinase

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    333
  • Mass (Da)
    34,831
  • Last updated
    2020-08-12 v1
  • Checksum
    9737941A99B5051D
MWTLPELPVQRELRRIAIITSGGDAPGMNAAVRAITRTAGSRGIETLGADRGYSGLVHEPLRPLSMSSVGSILQRGGTILKTDRCEAFMDPAVRLQTADALRAQGVDALIVIGGDGSLTGAHHFFADTGLPVLGLPGTIDNDIVGTDDSIGFDTAVNTALEAIDRIRDTAHSHDRLFLVEVMGRSSGFIALSVGIAGGAEAILLPDEAVNAETLLARMAQTRTREKASSLVVVAEGTDPTYTTRLAADLGAQGRPARACILGHIQRGGSPSGHDRVLASVLGASAVDHLQAGYSDIMLGIREGAIARTALERIGHERKKLPEAWLALASTLAS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP050695
EMBL· GenBank· DDBJ
QIT56813.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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