A0A6H0CRY4 · A0A6H0CRY4_9ACTN
- ProteinPhosphoenolpyruvate carboxykinase [GTP]
- GenepckG
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids607 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
Catalytic activity
- oxaloacetate + GTP = phosphoenolpyruvate + GDP + CO2
Cofactor
Note: Binds 1 Mn2+ ion per subunit.
Pathway
Carbohydrate biosynthesis; gluconeogenesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 80 | substrate | |||
Binding site | 222-224 | substrate | |||
Binding site | 231 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 251 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 273 | substrate | |||
Binding site | 274-279 | GTP (UniProtKB | ChEBI) | |||
Active site | 275 | ||||
Binding site | 298 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 388-390 | substrate | |||
Binding site | 390 | GTP (UniProtKB | ChEBI) | |||
Binding site | 421 | GTP (UniProtKB | ChEBI) | |||
Binding site | 516-519 | GTP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | GTP binding | |
Molecular Function | kinase activity | |
Molecular Function | manganese ion binding | |
Molecular Function | phosphoenolpyruvate carboxykinase (GTP) activity | |
Biological Process | cellular response to glucose stimulus | |
Biological Process | gluconeogenesis | |
Biological Process | glycerol biosynthetic process from pyruvate | |
Biological Process | phosphorylation | |
Biological Process | propionate catabolic process | |
Biological Process | response to lipid | |
Biological Process | response to starvation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhosphoenolpyruvate carboxykinase [GTP]
- EC number
- Short namesPEP carboxykinase ; PEPCK
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Kitasatosporales > Streptomycetaceae > Streptomyces
Accessions
- Primary accessionA0A6H0CRY4
Proteomes
Subcellular Location
Interaction
Subunit
Monomer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 21-243 | Phosphoenolpyruvate carboxykinase GTP-utilising N-terminal | |||
Domain | 247-604 | Phosphoenolpyruvate carboxykinase C-terminal P-loop | |||
Sequence similarities
Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length607
- Mass (Da)67,146
- Last updated2020-08-12 v1
- Checksum47D75869E8DCFC8E
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP050504 EMBL· GenBank· DDBJ | QIS72878.1 EMBL· GenBank· DDBJ | Genomic DNA |