A0A6H0CRY4 · A0A6H0CRY4_9ACTN

  • Protein
    Phosphoenolpyruvate carboxykinase [GTP]
  • Gene
    pckG
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 1 Mn2+ ion per subunit.

Pathway

Carbohydrate biosynthesis; gluconeogenesis.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site80substrate
Binding site222-224substrate
Binding site231Mn2+ (UniProtKB | ChEBI)
Binding site251Mn2+ (UniProtKB | ChEBI)
Binding site273substrate
Binding site274-279GTP (UniProtKB | ChEBI)
Active site275
Binding site298Mn2+ (UniProtKB | ChEBI)
Binding site388-390substrate
Binding site390GTP (UniProtKB | ChEBI)
Binding site421GTP (UniProtKB | ChEBI)
Binding site516-519GTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionGTP binding
Molecular Functionkinase activity
Molecular Functionmanganese ion binding
Molecular Functionphosphoenolpyruvate carboxykinase (GTP) activity
Biological Processcellular response to glucose stimulus
Biological Processgluconeogenesis
Biological Processglycerol biosynthetic process from pyruvate
Biological Processphosphorylation
Biological Processpropionate catabolic process
Biological Processresponse to lipid
Biological Processresponse to starvation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Phosphoenolpyruvate carboxykinase [GTP]
  • EC number
  • Short names
    PEP carboxykinase
    ; PEPCK
  • Alternative names
    • GTP-dependent phosphoenolpyruvate carboxykinase
      (GTP-PEPCK
      )

Gene names

    • Name
      pckG
    • ORF names
      HB370_25270

Organism names

  • Taxonomic identifier
  • Strain
    • DSM 40868
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Kitasatosporales > Streptomycetaceae > Streptomyces

Accessions

  • Primary accession
    A0A6H0CRY4

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain21-243Phosphoenolpyruvate carboxykinase GTP-utilising N-terminal
Domain247-604Phosphoenolpyruvate carboxykinase C-terminal P-loop

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    607
  • Mass (Da)
    67,146
  • Last updated
    2020-08-12 v1
  • Checksum
    47D75869E8DCFC8E
MARDNAAPTVVPTNHRELISWVNEIAELTQPDRVVWCDGSEAEYERLCEELVAKGTFKKLDPVKRPHSYYAASDPTDVARVEDRTFICSEKEEDAGPTNHWKAPAEMREIFTGDQGLFRGSMRGRTMYVVPFCMGPLGSPLSALGVEITDSAYVAVSMRTMTRMGQAVLDELGEEGFFVKAVHTLGAPLEPGQEDVPWPCNQTKYISHFPETREIWSYGSGYGGNALLGKKCYALRIASVMARDEGWLAEHMLILKLTPPQGEAKYVAAAFPSACGKTNLAMLEPTISGWTVETVGDDIAWMRFGEDGRLYAINPEAGFFGVAPGTGEHTNANAMKTLWGNAVFTNVALTDDGDVWWEGMTEEPPAHLTDWKGNDWTPGSGTPAAHPNARFTVPASQCPIIAPEWEDPKGVPISAILFGGRRATAVPLVTESFDWNHGVFLGANVASEKTAAAEGKVGELRRDPFAMLPFCGYNMGDYMAHWIDVAKDKDQSKLPKIYYVNWFRKDDEGRFVWPGFGENSRVLKWIVERLEGRAEGVETPIGILPAKGALDTDGLELPEADLDFLLTVDKEVWREEAALVPEHLNTFGDHTPKELWDQYHALLERLG

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP050504
EMBL· GenBank· DDBJ
QIS72878.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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