A0A6G8CIK3 · A0A6G8CIK3_9BURK

  • Protein
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • Gene
    ispH
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit.

Pathway

Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site16[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site45substrate
Binding site78substrate
Binding site100[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site128substrate
Active site130Proton donor
Binding site168substrate
Binding site198[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site226-228substrate
Binding site270substrate

GO annotations

AspectTerm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Function4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity
Molecular Functionmetal ion binding
Biological Processdimethylallyl diphosphate biosynthetic process
Biological Processisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • EC number
  • Short names
    HMBPP reductase

Gene names

    • Name
      ispH
    • Synonyms
      lytB
    • ORF names
      G7047_00180

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • HDW4A
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Comamonadaceae > Diaphorobacter

Accessions

  • Primary accession
    A0A6G8CIK3

Proteomes

Family & Domains

Sequence similarities

Belongs to the IspH family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    329
  • Mass (Da)
    35,799
  • Last updated
    2020-08-12 v1
  • Checksum
    8BE2FDD8F0C79AAD
MQKPQEIVLAEPRGFCAGVDRAIEIVERALQKFGRPIYVRHEIVHNTYVVNDLKNKGAIFIEELDDVPAGATLVFSAHGVSKAVQREAEARGFQIFDATCPLVTKVHVEVAKLAKEGYEFIMIGHKGHPEVEGTMGQLSEGIHLVEDLEDVVTVAPGQTERLAVVTQTTLSVDDAAAITAAVRARFPSIREPKQQDICYATQNRQDAVKLLSGQVDLVIVVGSPTSSNSNRLRELAARLGTPAYMIDSADELKHEWFDGIARVGLTAGASAPEVLVKDVIHRIKELGATSVRKMDGVEETLKFPLPKGLKIDAATGLEIEVRKAPETGR

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP049910
EMBL· GenBank· DDBJ
QIL78510.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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