A0A6G7BPP0 · A0A6G7BPP0_9SPHI

  • Protein
    tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
  • Gene
    miaB
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site41[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site77[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site111[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site186[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site190[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site193[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionmetal ion binding
Molecular Functionmethylthiotransferase activity
Biological ProcesstRNA methylthiolation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
  • EC number
  • Alternative names
    • (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
    • tRNA-i(6)A37 methylthiotransferase

Gene names

    • Name
      miaB
    • ORF names
      G6053_02350

Organism names

  • Taxonomic identifier
  • Strain
    • DR205
  • Taxonomic lineage
    Bacteria > Bacteroidota > Sphingobacteriia > Sphingobacteriales > Sphingobacteriaceae > Sphingobacterium

Accessions

  • Primary accession
    A0A6G7BPP0

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain32-148MTTase N-terminal
Domain172-412Radical SAM core
Domain414-478TRAM

Sequence similarities

Belongs to the methylthiotransferase family. MiaB subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    478
  • Mass (Da)
    54,379
  • Last updated
    2020-08-12 v1
  • MD5 Checksum
    9D501D7EFEC4436C9CF68C83D27F12DC
MLDLTHTTKEHDESRQGEALLLEQDPKISNGRKLYIESYGCQMNFSDSEIVASILLDKGFETTKNYQEADVVFINTCSIRENAEQRVRNRLKEFEAAKTKNPGMIVGVLGCMAERLKSKFLEEEKLVDVVVGPDAYRDLPNLIDKVDDGAKAVNVLLSREETYADINPVRLNSNGVTAFISIMRGCDNMCSFCVVPFTRGRERSRDPHSIVKEAQDLFNAGYKEVTLLGQNVDSYKHTATVAEGEAPAETVTFAKLLAMVAEVSPLLRVRFSTSHPKDITDEVLHTIASYENICNYIHLPVQSGNSRVLELMNRTYDREWYMERVDAIRKIIPDCAISTDVITGFCTETEAEHQETLSMMDYVKYDFAYMFAYSERPGTLAAKRYEDDIPEDVKKRRLTEVINKQRDHSLYRYQHFIGKVCKVLIESFSKRSEFDFCGRNDQNALVVFPIDPRYKQGDYVNVLVESCTSATLLGKIVD

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP049333
EMBL· GenBank· DDBJ
QIH31816.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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