A0A6G1F986 · A0A6G1F986_9ORYZ

Function

function

Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Pathway

Cofactor biosynthesis; molybdopterin biosynthesis.
tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site97ATP (UniProtKB | ChEBI)
Binding site118ATP (UniProtKB | ChEBI)
Binding site125-129ATP (UniProtKB | ChEBI)
Binding site142ATP (UniProtKB | ChEBI)
Binding site186-187ATP (UniProtKB | ChEBI)
Binding site227Zn2+ (UniProtKB | ChEBI)
Binding site230Zn2+ (UniProtKB | ChEBI)
Active site244Glycyl thioester intermediate; for adenylyltransferase activity
Binding site302Zn2+ (UniProtKB | ChEBI)
Binding site305Zn2+ (UniProtKB | ChEBI)
Active site423Cysteine persulfide intermediate; for sulfurtransferase activity

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionATP binding
Molecular Functionmetal ion binding
Molecular Functionmolybdopterin-synthase adenylyltransferase activity
Molecular Functionmolybdopterin-synthase sulfurtransferase activity
Molecular Functionubiquitin-like modifier activating enzyme activity
Biological ProcessMo-molybdopterin cofactor biosynthetic process
Biological ProcesstRNA wobble position uridine thiolation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Adenylyltransferase and sulfurtransferase MOCS3
  • Alternative names
    • Molybdenum cofactor synthesis protein 3

Including 2 domains:

  • Recommended name
    Molybdopterin-synthase adenylyltransferase
  • EC number
  • Alternative names
    • Adenylyltransferase MOCS3
    • Sulfur carrier protein MOCS2A adenylyltransferase
  • Recommended name
    Molybdopterin-synthase sulfurtransferase
  • EC number
  • Alternative names
    • Sulfurtransferase MOCS3
    • Sulfur carrier protein MOCS2A sulfurtransferase

Gene names

    • Name
      MOCS3
    • Synonyms
      CNX5
      , UBA4
    • ORF names
      E2562_018581

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Menghai
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza meyeriana

Accessions

  • Primary accession
    A0A6G1F986

Proteomes

Subcellular Location

Keywords

Family & Domains

Features

Showing features for coiled coil, domain.

TypeIDPosition(s)Description
Coiled coil12-39
Domain358-463Rhodanese

Sequence similarities

In the N-terminal section; belongs to the HesA/MoeB/ThiF family. UBA4 subfamily.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    465
  • Mass (Da)
    50,586
  • Last updated
    2020-08-12 v1
  • Checksum
    942AFC330D2B8130
MEDGGGCGRGRTEAIMRELERLRAERDELDGRIRLLESQLRVGASLPLGVSAEAETMGLPAADMIGRYSRHHLLPDFGLEGQRKLSQSSILVVGAGGLGSPVAMYLAACGVGCLGIVDGKQVEFDNLHRQIIHIEAYVGQPKVKAAAAACRVINSSVKVLDYHITLNPSNALDIVRQYDVVVDATDNLSSRYMISDCCVLMNKPLISGAAVGLEGQLTVYHHNGSPCYRCLDPNPPSSAALQGCSDNGILGILPGVIGCLQALEAIKVATAVGEPLCGRMLLFDALSSYVRIVKVYGSSPTCKVCGENPVFTKDDFVKFDYESFTQSPMSRKSTTPSLNLLPENARVSCRDYKRVVDSGRPHLLVDVRPSHHFRIASMAHSINIPLLLLEEKLPLLRDTAREVAESTSTRENSRQHCPLYVICRRGNDSQVAVQILRENGFLYASDVAGGFESWAREVDPSFPLY

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A6G1F9A9A0A6G1F9A9_9ORYZE2562_018581362

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
SPHZ02000001
EMBL· GenBank· DDBJ
KAF0933496.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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