A0A6F8V4L1 · A0A6F8V4L1_9HYPH
- ProteinCRISPR-associated endonuclease Cas9
- Genecas9
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1078 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity.
Cofactor
Features
Showing features for active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 24 | For RuvC-like nuclease domain | |||
Active site | 626 | Proton acceptor for HNH nuclease domain | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | DNA binding | |
Molecular Function | endonuclease activity | |
Molecular Function | metal ion binding | |
Molecular Function | RNA binding | |
Biological Process | defense response to virus | |
Biological Process | maintenance of CRISPR repeat elements |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCRISPR-associated endonuclease Cas9
- EC number
Gene names
Encoded on
- Plasmid panam02 dna
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Boseaceae > Bosea
Accessions
- Primary accessionA0A6F8V4L1
Proteomes
Interaction
Subunit
Monomer. Binds crRNA and tracrRNA.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 550-704 | HNH Cas9-type | |||
Domain
Has 2 endonuclease domains. The discontinuous RuvC-like domain cleaves the target DNA noncomplementary to crRNA while the HNH nuclease domain cleaves the target DNA complementary to crRNA.
Sequence similarities
Belongs to the CRISPR-associated Cas9 family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,078
- Mass (Da)119,794
- Last updated2020-08-12 v1
- Checksum71299A4B02290112
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP022849 EMBL· GenBank· DDBJ | BCB22357.1 EMBL· GenBank· DDBJ | Genomic DNA |