A0A6F8V4L1 · A0A6F8V4L1_9HYPH

  • Protein
    CRISPR-associated endonuclease Cas9
  • Gene
    cas9
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    2/5

Function

function

CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site24For RuvC-like nuclease domain
Active site626Proton acceptor for HNH nuclease domain

GO annotations

AspectTerm
Molecular FunctionDNA binding
Molecular Functionendonuclease activity
Molecular Functionmetal ion binding
Molecular FunctionRNA binding
Biological Processdefense response to virus
Biological Processmaintenance of CRISPR repeat elements

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    CRISPR-associated endonuclease Cas9
  • EC number

Gene names

    • Name
      cas9
    • ORF names
      OCUBac02_52510

Encoded on

  • Plasmid panam02 dna

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • ANAM02
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Boseaceae > Bosea

Accessions

  • Primary accession
    A0A6F8V4L1

Proteomes

Interaction

Subunit

Monomer. Binds crRNA and tracrRNA.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain550-704HNH Cas9-type

Domain

Has 2 endonuclease domains. The discontinuous RuvC-like domain cleaves the target DNA noncomplementary to crRNA while the HNH nuclease domain cleaves the target DNA complementary to crRNA.

Sequence similarities

Belongs to the CRISPR-associated Cas9 family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,078
  • Mass (Da)
    119,794
  • Last updated
    2020-08-12 v1
  • Checksum
    71299A4B02290112
MKRRVAISQGLLQHFYMGIRFSFDLGTNSIGWAVWRTGVDPRGIFGVDAPLEILRAGVRIFKDGRNPKSKASLASERRAARLARRTRDRFKLRREDLLKALAKHGLFPEAGEERLALAKLDPYELRARAVNEALATFEIGRALFHLHQRRGFKSQRKGVAQDEELGKIAAGTERLKIALAALGCSTLGEFLWQRRGERKSTRIRLDGAGNQALYEFYPTREIVQDEFQAIWSTQAAYHPAALTEVAKAEIEAILFRQRPIKPPPVGKCSFTDELRAPKALPSVAAREIYERLNHLRIGYDDGIRRPLSVAERDLLATELLRSAKLTFPRARKLLGLKAGVAFSLEEAGEKHIPGFKFAECVHMETAFGGRWDSLSWGEKDAIVSIVLAEEEDLDAAERLVAECSVPAPEARAIAAMIRVLPDGYSKLGLTANAAILEALATEVTADGSVVTFAEAVLRAGRAIGYDWHHSDQVRRSGRPAPEVSRQLPNYAVILPDQVMPALPGEEFGRFTNPTVHIGLNQLRKVVNELLIEFGHPSQIVVELARDLKQTEKQKKATAASNRANAKLNDKRRDLLQSLGVRVNGDSMLRLRLLDEQTGADGKARCPFSGQEITQAALFTGEIEVEHLLPMSRTLDDTAANKVVCVREKNREKRGRSPFEAFGDRAEWPEIWSRAKALPANKSWRFKPDAMERYEKSDGFLGRHLGETRHLSRIAQQYLQAICVRFDGATGERVYGDVFVTPGEMTALLRARWRLNELLGDTGSKNRDDHRHHAIDAIVIGTMTRGWLSHLACEAGRYEGGERADLVKAVAEPFPGFRDVVKAAVDRVVVSYKPEHGIEGALFKETAYGLVSEKHSDHRAGNVVYRKPLASISAKEVAKIRGQELRAGALGAIDGVDPENKKLIAAHLNRFGEEQGVRRARLLGVENAVVPKADAQGHEFTALKRDGIHHMDIVMLRDGSWKMYPAPITDVNRRGFRPSWEVQKVGGKLAMRLHKGDMIELDGDEGRVIKVVIKFSESGQIVVADHNAGGNLKTRDADKRDPFRYQSASAEKLRGLNAIALNVSPSGVMRPRRIDAGEI

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP022849
EMBL· GenBank· DDBJ
BCB22357.1
EMBL· GenBank· DDBJ
Genomic DNA

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Disclaimer

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