A0A6F8TM21 · A0A6F8TM21_9BACI

  • Protein
    3-isopropylmalate dehydrogenase
  • Gene
    leuB
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 1 Mg2+ or Mn2+ ion per subunit.
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site76-89NAD+ (UniProtKB | ChEBI)
Binding site96substrate
Binding site106substrate
Binding site134substrate
Site141Important for catalysis
Site190Important for catalysis
Binding site222Mg2+ (UniProtKB | ChEBI)
Binding site222substrate
Binding site246Mg2+ (UniProtKB | ChEBI)
Binding site250Mg2+ (UniProtKB | ChEBI)
Binding site280-292NAD+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function3-isopropylmalate dehydrogenase activity
Molecular Functionmagnesium ion binding
Molecular FunctionNAD binding
Biological ProcessL-leucine biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    3-isopropylmalate dehydrogenase
  • EC number
  • Alternative names
    • 3-IPM-DH
    • Beta-IPM dehydrogenase
      (IMDH
      )

Gene names

    • Name
      leuB
    • ORF names
      KH172YL63_04710

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • KH172YL63
  • Taxonomic lineage
    Bacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Bacillus

Accessions

  • Primary accession
    A0A6F8TM21

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain4-352Isopropylmalate dehydrogenase-like

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    370
  • Mass (Da)
    40,457
  • Last updated
    2020-08-12 v1
  • Checksum
    23BF23DE7AD82221
MKKKIAVLPGDGIGQEIMDGALEVLKAVGDWFGHEFEVEKGHIGGSAIDRYGVPLPPETIKLCKESDAVLLGAVGGPKWEHLPKEVRPEKGLLGIRKEFDLFANLRPVKAFESLLGASPLKREIVEEVDLVIVRELTGGLYFGQPSGRQGKQDEVAIDTLVYEKSEIERIVHKAFKLAQKRKKKVTSVDKANVLETSRMWREVVNEVAAEYPDVEVEHMLVDVAAMKLMYQPKQFDVLVTENMFGDILSDEASMITGSLGMLPSASLRGDGFGLYEPVHGSAPDIAGKGKANPLAMILSVAMMLRHSFGLKEEAEMIEMAVQEVLNAGYRTGDLWTMGRGTVVGTGAMSQLVVERLEADHALSSILAAYL

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP022842
EMBL· GenBank· DDBJ
BCB02338.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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