A0A6C1BUC0 · A0A6C1BUC0_9AQUI

Function

function

Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Fe2+ (UniProtKB | Rhea| CHEBI:29033 )

Note: Binds 1 Fe2+ cation per monomer.
Ni2+ (UniProtKB | Rhea| CHEBI:49786 )

Note: Binds 1 nickel ion per monomer.

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6.

Features

Showing features for site, binding site.

Type
IDPosition(s)Description
Site94May play a role in metal incorporation in vivo
Binding site95Fe2+ (UniProtKB | ChEBI)
Binding site95Ni2+ (UniProtKB | ChEBI)
Binding site97Fe2+ (UniProtKB | ChEBI)
Binding site97Ni2+ (UniProtKB | ChEBI)
Binding site101Fe2+ (UniProtKB | ChEBI)
Binding site101Ni2+ (UniProtKB | ChEBI)
Site103Important to generate the dianion
Binding site139Fe2+ (UniProtKB | ChEBI)
Binding site139Ni2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionacireductone dioxygenase (Ni2+-requiring) activity
Molecular Functionacireductone dioxygenase [iron(II)-requiring] activity
Molecular Functioniron ion binding
Molecular Functionnickel cation binding
Biological ProcessL-methionine salvage from methylthioadenosine
Biological ProcessL-methionine salvage from S-adenosylmethionine

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Acireductone dioxygenase
  • Alternative names
    • 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
      (DHK-MTPene dioxygenase
      )
    • Acireductone dioxygenase (Fe(2+)-requiring)
      (ARD'
      ; Fe-ARD
      ) (EC:1.13.11.54
      ) . EC:1.13.11.54 (UniProtKB | ENZYME | Rhea)
    • Acireductone dioxygenase (Ni(2+)-requiring)
      (ARD
      ; Ni-ARD
      ) (EC:1.13.11.53
      ) . EC:1.13.11.53 (UniProtKB | ENZYME | Rhea)

Gene names

    • Name
      mtnD
    • ORF names
      G3M65_06250

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • T-8
  • Taxonomic lineage
    Bacteria > Aquificota > Aquificia > Aquificales > Aquificaceae > Hydrogenobacter

Accessions

  • Primary accession
    A0A6C1BUC0

Proteomes

Interaction

Subunit

Monomer.

Family & Domains

Sequence similarities

Belongs to the acireductone dioxygenase (ARD) family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    184
  • Mass (Da)
    21,509
  • Last updated
    2020-06-17 v1
  • Checksum
    610645CEC134EBA4
MSLLVVYDEEGRLLEVERDYEKISQRLASIGIRFERWEAKAELPWSAGQEEVLEAYKEDVDRIVKEFDFKSIDVVSLTPEHPKKEELRNMFLSEHTHSDFEVRFFVDGCGTFYLHPEDKVYAVLCEKGDFISVPANTKHWFDMGTEPFFKAIRFFSIPDGWVANFTGSDISKKIPSHDHILKLR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP048795
EMBL· GenBank· DDBJ
QID33390.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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