A0A6C0WW38 · CKCS_CAMSB

Function

function

Involved in the biosynthesis of caffeine in cv. Puer (PubMed:32193380).
Involved in the biosynthesis of theacrine in cv. Kucha, a caffeine-like xanthine alkaloid with diverse beneficial biological activities including anti-depressive, sedative, and hypnotic activities, improving learning and memory, increasing exercise activity, and preventing nonalcoholic fatty liver disease (PubMed:32193380).
Catalyzes the conversion of 7-methylxanthine (7mX) to theobromine and of theobromine to caffeine (PubMed:32193380).
Has 3-N- and 1-N-methylation activity (PubMed:32193380).

Miscellaneous

Caffeine is catabolized to produce theacrine in Camellia sinensis var. assamica cv. Kucha, but not in cv. Puer.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
71.49 μM1,3,7-trimethyluric acid
kcat is 0.00012 sec-1 with 1,3,7-trimethyluric acid as substrate.

Pathway

Alkaloid biosynthesis.

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site24S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site24substrate
Binding site27-31substrate
Binding site65S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site65-66S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site66S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site71S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site101-104S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site138-140S-adenosyl-L-methionine (UniProtKB | ChEBI)
Site153Involved in substrate discrimination
Binding site155-157S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site156-160substrate
Binding site177Mg2+ (UniProtKB | ChEBI)
Site225Involved in substrate discrimination
Binding site263Mg2+ (UniProtKB | ChEBI)
Binding site265Mg2+ (UniProtKB | ChEBI)
Binding site266Mg2+ (UniProtKB | ChEBI)
Site269Involved in substrate discrimination
Site317Involved in substrate discrimination
Site332Involved in substrate discrimination

GO annotations

AspectTerm
Molecular Functioncaffeine synthase activity
Molecular Functionmetal ion binding
Molecular FunctionS-adenosylmethionine-dependent methyltransferase activity
Biological Processalkaloid metabolic process
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    3,7-dimethylxanthine N-methyltransferase TCS1
  • EC number
  • Alternative names
    • Caffeine synthase
      (CkCS
      )

Gene names

    • Name
      CS

Organism names

Accessions

  • Primary accession
    A0A6C0WW38

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004518011-3693,7-dimethylxanthine N-methyltransferase TCS1

Family & Domains

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    369
  • Mass (Da)
    41,577
  • Last updated
    2020-06-17 v1
  • Checksum
    9A1B80145FC301C3
MELATREKVNEVLFMNRGEGESSYAQNSSFTQQVASMAQPALENAVETLFSKDFHLQALNAADLGCAAGPNTFAVISTIKRMMEKKCRELNCQTLELQVYLNDLFGNDFNTLFKGLSSEVIGNKCEEVPCYVMGVPGSFHGRLFPRNSLHLVYSSYSVHWLTQAPKGLTSREGLALNKGKIYISKTSPPVVREAYLSQFHEDFTMFLNYRSQEMVPNGCMVLILRGRQCFDPSDMQSCFTWELLALAIAELVSQGLIDEDKLDTFNIPSYFASLEEVKDIVERDGSFTIDHIEGFDLDSVEMQENDKWVRGEKFTKVVRAFSEPIISSQFGHEIMDKLYDKFTHIVVSDLEAKLPKTTSIILVLSKIVG

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MN163829
EMBL· GenBank· DDBJ
QIC50342.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

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