A0A6C0WW36 · CKTCS_CAMSB

Function

function

Involved in the biosynthesis of theacrine, a caffeine-like xanthine alkaloid with diverse beneficial biological activities including anti-depressive, sedative, and hypnotic activities, improving learning and memory, increasing exercise activity, and preventing nonalcoholic fatty liver disease (PubMed:32193380).
Mediates the conversion of 1,3,7-trimethyluric acid to theacrine (PubMed:32193380).

Miscellaneous

Caffeine is catabolized to produce theacrine in Camellia sinensis var. assamica cv. Kucha, but not in cv. Puer.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
4.68 μM1,3,7-trimethyluric acid
kcat is 0.01142 sec-1 with 1,3,7-trimethyluric acid as substrate.

Pathway

Alkaloid biosynthesis.

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site24S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site24substrate
Binding site27-31substrate
Binding site66S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site66-67S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site67S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site72S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site102-105S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site139-141S-adenosyl-L-methionine (UniProtKB | ChEBI)
Site154Involved in substrate discrimination
Binding site156-158S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site157-161substrate
Binding site178Mg2+ (UniProtKB | ChEBI)
Site226Involved in substrate discrimination
Binding site264Mg2+ (UniProtKB | ChEBI)
Binding site266Mg2+ (UniProtKB | ChEBI)
Binding site267Mg2+ (UniProtKB | ChEBI)
Site270Involved in substrate discrimination
Site318Involved in substrate discrimination
Site333Involved in substrate discrimination

GO annotations

AspectTerm
Molecular Functionmetal ion binding
Molecular FunctionS-adenosylmethionine-dependent methyltransferase activity
Biological Processalkaloid metabolic process
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    1,3,7-trimethyluric acid N-methyltransferase CkTcS
  • EC number
  • Alternative names
    • Theacrine synthase
      (CkTcS
      )

Gene names

    • Name
      TCS

Organism names

Accessions

  • Primary accession
    A0A6C0WW36

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004518021-3701,3,7-trimethyluric acid N-methyltransferase CkTcS

Expression

Tissue specificity

Expressed in leaves of cv. Kucha, but not in cv. Puer.

Family & Domains

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    370
  • Mass (Da)
    41,606
  • Last updated
    2020-06-17 v1
  • Checksum
    49C72A7198F3C45B
MELATRGKVKEVLFMNTGEGESSYVQNSSFTEKVASMAMPALENAVETLFSKDFHLFQAINAADLGCATGPNTFAVISTIKRMMEKKCRELNCQTLELQVYMNDLFGNDFNTLFKGLSSKVIGNKCEEVSCYVMGVPGSFHGRLFPRNSLHLVHSSYSVHWLTQAPKGLTSREGLALNKGRIYISKTSPPVVREAYLSQFHEDFTMFLNARSQEVVPNGCMVLILRGRQSSDPSDMQSCFIWELLAIAIAELVSQGLIDEDKLDTFNIPCYFPSLEEVKDIVERDGSFTIDHMEGFELDSLQMQENDKWVRGEKFAKIVRAFTEPIISNQFGHEIMDKLYDKFTHIVVSDLEAKLPKTTSIILVLSKIVG

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MN163831
EMBL· GenBank· DDBJ
QIC50344.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

Disclaimer

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