A0A6C0MAZ0 · A0A6C0MAZ0_9GEMI

  • Protein
    Replication-associated protein
  • Gene
    rep
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific region at the genome origin of replication. It introduces an endonucleolytic nick within the conserved sequence 5'-TAATATTAC-3' in the intergenic region of the genome present in all geminiviruses, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the + strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities.

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Divalent metal cations, possibly Mg2+ or Mn2+.
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site48a divalent metal cation (UniProtKB | ChEBI)
Binding site56a divalent metal cation (UniProtKB | ChEBI)
Binding site58a divalent metal cation (UniProtKB | ChEBI)
Active site102For DNA cleavage activity
Binding site106a divalent metal cation (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componenthost cell nucleus
Molecular FunctionATP binding
Molecular FunctionDNA binding
Molecular Functionendodeoxyribonuclease activity, producing 5'-phosphomonoesters
Molecular Functionhelicase activity
Molecular Functionmetal ion binding
Molecular Functionnucleotidyltransferase activity
Molecular Functionstructural molecule activity
Biological ProcessDNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Replication-associated protein
  • EC number
  • Short names
    Rep

Gene names

    • Name
      rep

Organism names

  • Taxonomic identifier
  • Organism
  • Strains
    • Cacti_2_1
    • SI_0_1
    • SI_0_3
    • SI_0_4
    • SI_7_1
  • Taxonomic lineage
    Viruses > Monodnaviria > Shotokuvirae > Cressdnaviricota > Repensiviricetes > Geplafuvirales > Geminiviridae > Opunvirus

Accessions

  • Primary accession
    A0A6C0MAZ0

Subcellular Location

Keywords

PTM/Processing

Keywords

Interaction

Subunit

Homooligomer. Rep binds to repeated DNA motifs (iterons). Forms the O-complex, which is a Rep-DNA complex involved in the initiation of RCR. Part of the C- and V-complexes which are RepA-Rep-DNA complexes involved in the c-sense and v-sense transcription.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain7-115CRESS-DNA virus Rep endonuclease

Domain

There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.

Sequence similarities

Belongs to the geminiviridae Rep protein family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    349
  • Mass (Da)
    40,427
  • Last updated
    2020-06-17 v1
  • Checksum
    7A16F947DD2749FA
MRNTKFSINAKNYFLTFPNCSLSKEEALSQLKSLKTPTNIKFIRICEEHHQDGSPHLHVLIQFEKKFQCKNQRFFDLRHPSSSIQFHPNIQSAKSSSDVKLYMEKERCFVDFGEFQIDPRSARGGKQSSNESYAKALNASSKEEALQIIKEEQPQHYVLMFHNINSNLDRIFTQPPQQFENKFSNFKITDDMEQWLEETFEFDVTSPAGPIIHKIIEDRPKSLIVEGPSRTGKTAWARSLGAHNYLAGHLDFNSKCFSNSAMYNVIDDVTPHYLKLKHWKELIGAQRDWQSNCKYGKPVQIKGGIPSIVLCNPGPETSYKEFLDKEENRALRDWTLQNAHFIFITQPLF

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MN099988
EMBL· GenBank· DDBJ
QHU79383.1
EMBL· GenBank· DDBJ
Genomic DNA
MN100019
EMBL· GenBank· DDBJ
QHU79569.1
EMBL· GenBank· DDBJ
Genomic DNA
MN100021
EMBL· GenBank· DDBJ
QHU79581.1
EMBL· GenBank· DDBJ
Genomic DNA
MN100022
EMBL· GenBank· DDBJ
QHU79587.1
EMBL· GenBank· DDBJ
Genomic DNA
MN100027
EMBL· GenBank· DDBJ
QHU79617.1
EMBL· GenBank· DDBJ
Genomic DNA
MN100029
EMBL· GenBank· DDBJ
QHU79629.1
EMBL· GenBank· DDBJ
Genomic DNA
MN100030
EMBL· GenBank· DDBJ
QHU79635.1
EMBL· GenBank· DDBJ
Genomic DNA
MN100031
EMBL· GenBank· DDBJ
QHU79641.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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