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A0A6B9TKE0 · A0A6B9TKE0_9EURY

Function

function

Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. tRNA 3'-terminal CCA addition is required both for tRNA processing and repair. Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs. While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded.

Miscellaneous

A single active site specifically recognizes both ATP and CTP and is responsible for their addition.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site49ATP (UniProtKB | ChEBI)
Binding site49CTP (UniProtKB | ChEBI)
Binding site52ATP (UniProtKB | ChEBI)
Binding site52CTP (UniProtKB | ChEBI)
Binding site61Mg2+ (UniProtKB | ChEBI)
Binding site63Mg2+ (UniProtKB | ChEBI)
Binding site116Mg2+ (UniProtKB | ChEBI)
Binding site139ATP (UniProtKB | ChEBI)
Binding site139CTP (UniProtKB | ChEBI)
Binding site159ATP (UniProtKB | ChEBI)
Binding site159CTP (UniProtKB | ChEBI)
Binding site168ATP (UniProtKB | ChEBI)
Binding site168CTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionAMPylase activity
Molecular FunctionATP binding
Molecular FunctionCCA tRNA nucleotidyltransferase activity
Molecular Functionmagnesium ion binding
Molecular FunctiontRNA binding
Biological ProcessRNA repair
Biological ProcesstRNA 3'-terminal CCA addition

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    CCA-adding enzyme
  • EC number
  • Alternative names
    • CCA tRNA nucleotidyltransferase
    • tRNA CCA-pyrophosphorylase
    • tRNA adenylyl-/cytidylyl- transferase
    • tRNA nucleotidyltransferase
    • tRNA-NT

Gene names

    • Name
      cca
    • ORF names
      FZP68_04980

Organism names

  • Taxonomic identifier
  • Strain
    • THM-2
  • Taxonomic lineage
    Archaea > Euryarchaeota > Methanomada group > Methanobacteria > Methanobacteriales > Methanobacteriaceae > Methanothermobacter

Accessions

  • Primary accession
    A0A6B9TKE0

Proteomes

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain33-139Polymerase nucleotidyl transferase
Domain153-263tRNA nucleotidyltransferase substrate binding
Domain282-422CCA-adding enzyme C-terminal

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    452
  • Mass (Da)
    51,599
  • Last updated
    2020-06-17 v1
  • MD5 Checksum
    6CE8CCD7EB8CF118CDC6203EDA472091
MDYSEILESIKPRGEEYQRVMELSESLVECINELAASRGIDAEAFLVGSVAKGTWLSGGADIDIFIHFPMDTPEDELKEKGLKLGYLCIEMFRGEAEERYASHPYVTGHIDGYEVDFVPCYRISHASELRSAVDRTILHTEYIQRNLKEEQMDEVLLLKQFMKSTGTYGSEFRVGGFAGYLAELLVLHYGDFEGVLKGAITWRPGYIIDLEGHGTGGGFQEPLVVVDPVDRNRNVAAALTLQRMAEFVTAARNFLRDPKPEYFRKPQYTSDPGEILETIEKRGSKVILVSSGVPDVPADALYPQLRKTLDSIVRNLENEGFSVLGADYWSDESSVAVMLIEMEVWALPSYRKRYGPPVWSRRHSERFTERHERVWVEGPRLVVESPRRDRNAVDYIKRLLSKPERLKMGKHIRKELINGFSVDFLENTLGSLDPQFLMFLDAFLNPWKAIRR

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP043480
EMBL· GenBank· DDBJ
QHN08145.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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