A0A6B9PS92 · A0A6B9PS92_9GEMI

Function

function

Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific region at the genome origin of replication. It introduces an endonucleolytic nick within the conserved sequence 5'-TAATATTAC-3' in the intergenic region of the genome present in all geminiviruses, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the + strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities.

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Divalent metal cations, possibly Mg2+ or Mn2+.
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site.

119520406080100120140160180
TypeIDPosition(s)Description
Binding site51a divalent metal cation (UniProtKB | ChEBI)
Binding site59a divalent metal cation (UniProtKB | ChEBI)
Binding site61a divalent metal cation (UniProtKB | ChEBI)
Active site105For DNA cleavage activity
Binding site109a divalent metal cation (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componenthost cell nucleus
Molecular FunctionATP binding
Molecular FunctionDNA binding
Molecular Functionendodeoxyribonuclease activity, producing 5'-phosphomonoesters
Molecular Functionhelicase activity
Molecular Functionmetal ion binding
Molecular Functionnucleotidyltransferase activity
Molecular Functionstructural molecule activity
Biological ProcessDNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Replication-associated protein
  • EC number
  • Short names
    Rep

Organism names

  • Taxonomic identifier
  • Strain
    • BR-Am-02
  • Taxonomic lineage
    Viruses > Monodnaviria > Shotokuvirae > Cressdnaviricota > Repensiviricetes > Geplafuvirales > Geminiviridae > Begomovirus

Accessions

  • Primary accession
    A0A6B9PS92

Subcellular Location

Keywords

PTM/Processing

Keywords

Interaction

Subunit

Homooligomer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain9-118CRESS-DNA virus Rep endonuclease

Domain

There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.

Sequence similarities

Belongs to the geminiviridae Rep protein family.

Family and domain databases

Sequence

  • Sequence status
    Fragment
  • Length
    195
  • Mass (Da)
    21,999
  • Last updated
    2020-06-17 v1
  • Checksum
    335315B68A8FD7F5
MPRNPNSFRLTAKNIFLTYPRCDIPKDEVLQLLRDLPWAVVKPTYIRVARELHADGFPHLHCLIQLSGKSNIKDARFFNLTHPRRSAEFHPNAQSAKDANAVKNYITKEGDYCESGQYKVSGGSKSNKDDVYHNAVNAATAGEALDIIRAGDPKTFIVNYHNVKANVERLFIKPPLPWVPPFQLSSFNNVPSDLQ

Features

Showing features for non-terminal residue.

TypeIDPosition(s)Description
Non-terminal residue195

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MG773279
EMBL· GenBank· DDBJ
QHD59439.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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