A0A6B8JMJ9 · A0A6B8JMJ9_9GAMM

  • Protein
    DNA topoisomerase 1
  • Gene
    topA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
    EC:5.6.2.1 (UniProtKB | ENZYME | Rhea)

Features

Showing features for site, active site.

1803100200300400500600700800
TypeIDPosition(s)Description
Site33Interaction with DNA
Site143Interaction with DNA
Site144Interaction with DNA
Site147Interaction with DNA
Site159Interaction with DNA
Active site323O-(5'-phospho-DNA)-tyrosine intermediate
Site325Interaction with DNA
Site518Interaction with DNA

GO annotations

AspectTerm
Molecular FunctionDNA binding
Molecular FunctionDNA topoisomerase type I (single strand cut, ATP-independent) activity
Molecular Functionmetal ion binding
Biological ProcessDNA topological change

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA topoisomerase 1
  • EC number
  • Alternative names
    • DNA topoisomerase I

Gene names

    • Name
      topA
    • ORF names
      GJ672_00325

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • 2438
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Chromatiales > Ectothiorhodospiraceae > Spiribacter

Accessions

  • Primary accession
    A0A6B8JMJ9

Proteomes

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain3-118Toprim
Region167-172Interaction with DNA
Region738-803Disordered
Compositional bias741-757Basic and acidic residues

Sequence similarities

Belongs to the type IA topoisomerase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    803
  • Mass (Da)
    89,752
  • Last updated
    2020-06-17 v1
  • Checksum
    DA75C8ECB19215BC
MSTKLVIVESPAKARTINKYLGNDYEVMASYGHVRDLVAREGAVDPEQGFEMHYTVVERNAKQVDAIAKATRKAEAVYLATDPDREGEAISWHLHELLRERGVLDGKPVHRVVFYEITKGAIQEAIEHPRGLSDDLVNAQQARRALDYLVGFNLSPLLWRKITSGLSAGRVQSPALRMIVEREQEIEAFVPREYWSVEADLQREGHDFVGRLQQLDGGKVEQFTITDGETAETAGRRILAAAGAASDTPLGEPLPESGRGELVVTRVEKKQRRRNPSAPFITSTLQQEASRKLRFSAQQTMRIAQQLYEGVDTGSGTVGLITYMRTDSVNLSQEAIQSLRAVIEKRFGPEKLPKQAHQYRTRAKNAQEAHEGIRPTDAARAPEQLKGYLSDDQYRLYDLIWKRTVACQMIHATIDTVSVDLAAGEVANFRATGSTIRDPGFMAVYQDGGDDRAADGNERILPPLSEGDRIPLLALRPEQHFTEPPPRYSEASLVRALEEYGIGRPSTYAAIISTLQTRNYVTLENRRFQPTDTGRVVARFLTEHFDRYVDYEFTAKLEDRLDAVSRGENDWVPVLEEFWKPFRERVEEKQQVSRSDVMKARELGTDPKSGRPVSARLGRYGPFAQIGTRDDEEKPKFASLRPGQSIETITLEEALALFTLPRDLGTTPDGLPLSVNIGRFGPYVRFGDQFASLKKDDDPYSIDRETALQRVEEKKENIAKRTIQSFDDGALTVMKGRFGPYVTDGRKRARVPKDQDAESLTKAQCQTLVDEAPEPKGRGGTKRKSTSGRKTASKGGGKRSSGS

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias741-757Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP046046
EMBL· GenBank· DDBJ
QGM20871.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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