A0A6A1UNE5 · A0A6A1UNE5_9ROSI
- ProteinCytoplasmic tRNA 2-thiolation protein 1
- GeneNCS6
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids356 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
Pathway
tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 67-69 | ATP (UniProtKB | ChEBI) | |||
Binding site | 73 | ATP (UniProtKB | ChEBI) | |||
Binding site | 99 | ATP (UniProtKB | ChEBI) | |||
Binding site | 177 | ATP (UniProtKB | ChEBI) | |||
Binding site | 182 | ATP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosolic tRNA wobble base thiouridylase complex | |
Cellular Component | mitochondrion | |
Molecular Function | ATP binding | |
Molecular Function | nucleotidyltransferase activity | |
Molecular Function | tRNA binding | |
Biological Process | protein urmylation | |
Biological Process | tRNA wobble position uridine thiolation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCytoplasmic tRNA 2-thiolation protein 1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fagales > Myricaceae > Morella
Accessions
- Primary accessionA0A6A1UNE5
Proteomes
Subcellular Location
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 64-247 | tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal | |||
Domain | 291-321 | Cytoplasmic tRNA 2-thiolation protein 1 C-terminal | |||
Region | 329-356 | Disordered | |||
Sequence similarities
Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length356
- Mass (Da)40,478
- Last updated2020-06-17 v1
- MD5 Checksum85B0C1696DACB7FC5CFA3CD0EEE4B454
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
RXIC02000027 EMBL· GenBank· DDBJ | KAB1201723.1 EMBL· GenBank· DDBJ | Genomic DNA |