A0A678T4E1 · A0A678T4E1_9POTV

Function

function

An RNA-dependent RNA polymerase that plays an essential role in the virus replication.
Has RNA-binding and proteolytic activities.
Has helicase activity. It may be involved in replication.
Indispensable for virus replication.
Involved in aphid transmission, cell-to-cell and systemis movement, encapsidation of the viral RNA and in the regulation of viral RNA amplification.
Mediates the cap-independent, EIF4E-dependent translation of viral genomic RNAs. Binds to the cap-binding site of host EIF4E and thus interferes with the host EIF4E-dependent mRNA export and translation. VPg-RNA directly binds EIF4E and is a template for transcription. Also forms trimeric complexes with EIF4E-EIF4G, which are templates for translation.
Required for aphid transmission and also has proteolytic activity. Only cleaves a Gly-Gly dipeptide at its own C-terminus. Interacts with virions and aphid stylets. Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May have RNA-binding activity.

Catalytic activity

  • ATP + H2O = ADP + phosphate + H+
    EC:3.6.4.13 (UniProtKB | ENZYME | Rhea)
  • Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.
    EC:3.4.22.45 (UniProtKB | ENZYME | Rhea)
  • Hydrolyzes glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or Gly) for the enzyme from tobacco etch virus. The natural substrate is the viral polyprotein, but other proteins and oligopeptides containing the appropriate consensus sequence are also cleaved.
    EC:3.4.22.44 (UniProtKB | ENZYME | Rhea)

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site633For helper component proteinase activity
Active site706For helper component proteinase activity

GO annotations

AspectTerm
Cellular Componenthelical viral capsid
Cellular Componenthost cell cytoplasmic vesicle
Cellular Componenthost cell nucleus
Cellular Componentvesicle
Molecular FunctionATP binding
Molecular Functioncysteine-type endopeptidase activity
Molecular Functionhelicase activity
Molecular Functionhydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
Molecular FunctionRNA binding
Molecular FunctionRNA-dependent RNA polymerase activity
Molecular Functionstructural molecule activity
Biological ProcessDNA-templated transcription
Biological Processproteolysis
Biological ProcessRNA-protein covalent cross-linking
Biological Processviral RNA genome replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Genome polyprotein
  • EC number

Organism names

  • Taxonomic identifier
  • Organism
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Pisuviricota > Stelpaviricetes > Patatavirales > Potyviridae > Potyvirus > Potyvirus yiornithogali

Accessions

  • Primary accession
    A0A678T4E1

Subcellular Location

Keywords

PTM/Processing

Keywords

Interaction

Subunit

Interacts with host eIF4E protein (via cap-binding region); this interaction mediates the translation of the VPg-viral RNA conjugates. Part of a complex that comprises VPg, RNA, host EIF4E and EIF4G; this interaction mediates the translation of the VPg-viral RNA conjugates.

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain140-288Peptidase S30
Domain625-747Peptidase C6
Domain1220-1372Helicase ATP-binding
Domain1391-1550Helicase C-terminal
Domain2025-2244Peptidase C4
Domain2511-2635RdRp catalytic
Region2786-2831Disordered
Compositional bias2792-2823Basic and acidic residues

Sequence similarities

Belongs to the potyviridae genome polyprotein family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    3,055
  • Mass (Da)
    344,248
  • Last updated
    2020-06-17 v1
  • Checksum
    F9BE086EE3225892
MAGAVHTMGANNQPEYELNFHPIGHHWVFTVDKVNKQVEQPTLTATKLDVLDEWERAYVARRFKGEVRAVTPAANFNRVQRQKAARLATLEAQKQELAEFRAQPTHIITQIHVPQVIAKVDASPGATKVVVSRAAQKITKPRTKRVTNQVRVFVGLMRVLRERELSVEVRDSGRRAALFARRKRVPYNALHVATRHERGHLDQVDVSCTPWSFKVLATLSKISSLKQGQNWKAPLGPGCSGMIVPEGTYTFGAQTVVGPTIIRGAIGRTIVSAEKMLEWDHVLQTTHFSDPAQPFWEGFDTTYRSTRRPPRTHTSCERTLSVRDVGSEEALLRLAIAPSFKITCAVCKQDLANTSLASYRSEALPRIDGIIEQLETIPSGSEHIQTALSYIRTALASYISDPSALLQARAMVGAKHEGPFKLLAQLCDLALRIGELDAGGCNQLAQTVLELTRWCVNRTDSIKRGDLSTFRNKASGKAHVNLALMCDNQLDANGNFLWGERSYHARRFFSNFFEEIEPGSGYAKYLLRKHPAGERKLAIGNLVVSLDLKQLRTQLTGDKIETPPLTQQCVSMRDAQFAYHCSCVTREDGTPQSSGVITPSKNHLVIGTTGDAKYVDLPPNPSRKLYIAKEGYCYMNIFLAMLVNVPEGEAKDFTKRVRDTLVPQLGEWPTMQQLATACYFLTIFHPETRSAELPRILVDHATSTMHVIDSYGSLTTGYHILKANSVSQLIDFAHDDMMSTMKTYRVGGLVRGSNKEVLKLLIRAVYRPDLLKQILLDEPYILLLSVLSPRILRAMADSGAFEYAVEHWIERNQTVAGILSQMRLLAQRCTVARMLYQQQLTIEREAQRLLLLVQGCSHFSESKLLVQAQLDLIARSYLTDLQLTTGGFQATDDFLLQSVEKNYDAMLIQSWTELTFLERLQYMVHRRKYYRVCVVDSQQTRLADLRDKLVTFADYSALEARERASSTARWMRGRVRRVQYGFKRFCVRGVLALVWRALPKHLRLVDTLTVAALILAIIHRIRKIVISHKKARWLKAKSDYKQHLVEFEDIVDRLDKSLGRDASGEEIARHVERYYPHLQNMLGRHVDDVEFQAKRTANEAYLERTVALIALGTMIFGSECSDGVFRTLNKLKAVFSTLHESVTFESASVEDSTTVEEDKLLTIDFETEQEVRPTIAQMDATFGTWWDHQITNGNTCPNYRASGTFIEFTRESAELVAANIAHATDTKEFLVRGAVGSGKSTGLPTYLSKRGKVLVLEPTRPLAENVHAQLSREPFFMSPTLRMRNHSRFGSSPITVMTSGFALHLFSNNPGQLSSYDFIIFDECHVMDASAMAFYCVLREYTFAGKILKVSATPPGRECEFTTQKPVEIRCEETLSFESFVKAQRTGSNSDVVRNGRNILVYVASYNDVDRLSRLLLEAGFAARKVDGRTMKSGVVTIDCTGTTEKPLFIVATNIIENGVTIDVDVVVDFGEKVVAELDVDNRGMAYKKVPVSFGERIQRLGRVGRFKAGTALRIGHTERGAQETTQAIATEAAFLCFAYGLPVITHNVTTSMLDKCTSKQARTMLQFELPVHYMLDLVRHDGMMHPAIYAVLKQYKLRESETVLHVGASPRLASKRWLTGEQYSRRGFRNNMDAHVRAPFLASGIPDTLHAKLWEAIDSCKDVEHFRSLSSASACKIAYTLQTDVFAIPRTLGTIEQLIREEQTKHACFETTRAQPIDAQGGTLTAIFNLLRSKMSVDHSMENIRRLEAARGQLLEFASLDYDLSKPELLRQFGSLTTVLFQSANDVASAVQLKGRWMGTLNTTNLVVVGALAVGGVAMLYQYFTRACEEKVEFQGFAKRQRQKLKFRNARDARMGREVFGEDAVEYHFGDAYTKKEKKKGKKVGMGIKNRRFVNFYGFDPTDYSIVRFVDPLTGHTLDESTTCDIQMIQDELYDRRIGAVQRDELSKEAMSYHTTIQAYFLKHGSDVALKVDLTPHLPLKVCEGSNTIAGFPERQGELRQTGRATQVSITDVPGSNKEVELEGASVYGGPRDYNPVANAICQLVNKSEGAGYQMYGVGYGPLIITSAHFFMHGNSGEMQVRSKHGLFKVPCIKSLRVQQVEGRDLVVIWMPKDFPPFPSKLKFTSPTKGSKVCMVNTIFNTGTSTSEVSESCMTFPKENTGFWMHWISTNGGHCGLPFVNTATGAIVGLHSLSSKTTVVNYYSGIPERFSEHFLAQPTVHEWTRDWQFNLDLINWGGMNVVNAAPTGSFRPTKVVTALPEEVVVQGFEHQSSWLEPHIKDNLQIVAKCPNQLVTKHIVKGLCPHFQLYLSLHPDAEAYFRPLMGAYSKSPLNRSAFIKDFTKYSGAIEIGTVDVEAFARAVSNVETLLRSLGLDECEYVNDSETIFSSLNMKAAVGALYTGKKQEYFEGYTAEQLEDTLRESCERVFNGKFGVWNGSLKAELRPIEKVLANKTRVFTAAPLDSLLANKMCVDDFNNRFYEKHLEGPWTVGISKFYRGWDRLMKSLPDGWAYCDADGSRFDSSLTPLLLNAVLQLRLQMMEDWDLGEKLLRNLYAEIVYTPIATPDGSIVKKFKGNNSGQASTVVDNSLMVVLTMQYALERLGFRYEEQNEFIRYFVNGDDLIIAVEPKHVWILDKLQDSFRELGLDFDFSARTESRENLSYMSHQGLLRDGVYIPKICKERIVSILEWDRSGSPEQRLDAICAAQIEAWGYDELLHEVRKFYSWLIGVEPFRQIAAEGGAPYVAESALRYLYLGEELTMSELERYVLEYEQRLGEVKAEEVVEFQSGTQDAGDEAERKRKADEKAQRENENKQRSEGRDKDVNSSTSGTYVVPRLRRMTKMRVPKFGGTDLLDLGHLLTYKPDEISLSNTRATQAQFERWCNQVMEAYQVTADEFRIIANGLMVWCVENGTSPNINGNWIMMDGKEQVEYPLKPVVEFAKPTLRQIMAHFSDAAEAYIEMRNAEEPYMPRYGLMRNLTDMSLARYAFDFYEVHARTPVRAREAHMQMKAAALRGSRSRLFGLDGNVGDASEDTERHTADDVNRNMHNLMGLKM

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias2792-2823Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KY711418
EMBL· GenBank· DDBJ
AVV48515.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

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