A0A674HU15 · A0A674HU15_TAEGU

Function

function

Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer). Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine. May be involved in lipid droplet biogenesis at the endoplasmic reticulum membrane.

Catalytic activity

Cofactor

pyruvate (UniProtKB | Rhea| CHEBI:15361 )

Note: Binds 1 pyruvoyl group covalently per subunit.

Pathway

Lipid metabolism.
Phospholipid metabolism; phosphatidylethanolamine biosynthesis.

Features

Showing features for active site, site.

Type
IDPosition(s)Description
Active site205Charge relay system; for autoendoproteolytic cleavage activity
Active site281Charge relay system; for autoendoproteolytic cleavage activity
Site391-392Cleavage (non-hydrolytic); by autocatalysis
Active site392Charge relay system; for autoendoproteolytic cleavage activity
Active site392Schiff-base intermediate with substrate; via pyruvic acid; for decarboxylase activity

GO annotations

AspectTerm
Cellular Componentlipid droplet
Cellular Componentmitochondrial inner membrane
Molecular Functionphosphatidylserine decarboxylase activity
Biological Processphosphatidylethanolamine biosynthetic process
Biological Processprotein autoprocessing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

Gene names

    • Name
      PISD

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Archelosauria > Archosauria > Dinosauria > Saurischia > Theropoda > Coelurosauria > Aves > Neognathae > Neoaves > Telluraves > Australaves > Passeriformes > Passeroidea > Estrildidae > Estrildinae > Taeniopygia

Accessions

  • Primary accession
    A0A674HU15

Proteomes

Subcellular Location

Phosphatidylserine decarboxylase alpha chain

Mitochondrion inner membrane
; Peripheral membrane protein
Note: Anchored to the mitochondrial inner membrane through its interaction with the integral membrane beta chain.

Phosphatidylserine decarboxylase beta chain

Mitochondrion inner membrane
; Single-pass membrane protein

Features

Showing features for topological domain.

TypeIDPosition(s)Description
Topological domain1-68Mitochondrial matrix
Topological domain88-423Mitochondrial intermembrane

Keywords

PTM/Processing

Features

Showing features for chain, modified residue.

Type
IDPosition(s)Description
ChainPRO_50257428381-391Phosphatidylserine decarboxylase beta chain
Modified residue392Pyruvic acid (Ser); by autocatalysis
ChainPRO_5025742837392-423Phosphatidylserine decarboxylase alpha chain

Post-translational modification

Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The autoendoproteolytic cleavage occurs by a canonical serine protease mechanism, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl prosthetic group on the alpha chain. During this reaction, the Ser that is part of the protease active site of the proenzyme becomes the pyruvoyl prosthetic group, which constitutes an essential element of the active site of the mature decarboxylase.

Keywords

Interaction

Subunit

Heterodimer of a large membrane-associated beta subunit and a small pyruvoyl-containing alpha subunit.

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region32-60Disordered

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    423
  • Mass (Da)
    47,803
  • Last updated
    2020-06-17 v1
  • MD5 Checksum
    161486911D1C564AC7D901DC63901CA2
MVRYYKALSNPPPSCYNLHKVKIHVRRLRSGNGGSSSCAGDQHPQLGSPGPAGSAGGTPSRRTRFRLQFPQLALRRRLGQLSCMSRPALKLRSWPLTILYYLLPFGALKPLTRVGWRPVSRVALYKSVPTRLLSRAWGRLNQVELPTWLRKPVYSLYIWTFGVNMKEAAVEDLHHYRNLSEFFRRKLKPQARPVCGVHSVISPSDGKILNFGQVKNCEVEQVKGVTYSLESFLGPRISTDEMHFSQAPPGNTFQQQLVTKEGNELYHCVIYLAPGDYHCFHSPTDWTVSHRRHFPGSLMSVNPGVARWIKELFCHNERVVLTGDWKHGFFSLTAVGATNVGSIRIYFDQDLHTNSPSYSKGSYNDFSFISNNNKEGIPMRKGEHLGEFNLGSTIVLIFEAPKDFRFNLKAGQKIHFGEALGSL

Computationally mapped potential isoform sequences

There are 2 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
H0ZHR7H0ZHR7_TAEGUPISD422
A0A674HUF0A0A674HUF0_TAEGUPISD401

Keywords

Sequence databases

Genome annotation databases

Similar Proteins

Disclaimer

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