A0A654EZU9 · A0A654EZU9_ARATH
- ProteintRNA 4-demethylwyosine synthase (AdoMet-dependent)
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids647 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Probable component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine, an intermediate in wybutosine biosynthesis.
Catalytic activity
- N1-methylguanosine37 in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine37 in tRNA(Phe) + 5'-deoxyadenosine + CO2 + H2O + L-methionine
RHEA-COMP:10165 CHEBI:73542 Position: 37+ CHEBI:15361 + CHEBI:59789 = RHEA-COMP:10164 CHEBI:64315 Position: 37+ CHEBI:17319 + CHEBI:16526 + CHEBI:15377 + CHEBI:57844
Cofactor
Pathway
tRNA modification; wybutosine-tRNA(Phe) biosynthesis.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | FMN binding | |
Molecular Function | metal ion binding | |
Molecular Function | oxidoreductase activity, acting on NAD(P)H | |
Molecular Function | tRNA-4-demethylwyosine synthase activity | |
Biological Process | tRNA processing |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA 4-demethylwyosine synthase (AdoMet-dependent)
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionA0A654EZU9
Proteomes
Expression
Gene expression databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 50-198 | Flavodoxin-like | ||||
Sequence: GKIFFISQTGTAKALAQRLHELCASNDIAFDIVDPHSYEPEDLPKETLVLFIASTWDGGKPPKNGEFLVNWLGESAEDFRVGSLLLSDCKFAVFGVGSRAYGESYNAVAKEMSSRMIGLGGLEMIPVGEGDVDDGELDRAFQDWCDGVI | ||||||
Domain | 316-559 | Radical SAM core | ||||
Sequence: YGIESHRCMETTPSLACANKCVFCWRHHTNPVGKSWQWKMDEPSVIVKGALDLHKNMIKQMKGVPGVTPEKLQEGLNPRHCALSLVGEPIMYPEINALVDELHGRRISTFLVTNAQFPEKILMMKPITQLYVSVDAATKESLKAIDRPLFADFWERFIDSLKALQEKQQRTVYRLTLVKGWNTEELDAYFNLFSIGKPDFIEIKGVTYCGSSATSKLTMENVPWHTDVKAFSEALSLKSNGEYE |
Sequence similarities
Belongs to the TYW1 family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length647
- Mass (Da)72,098
- Last updated2020-04-22 v1
- Checksum2E0D968DF64CA4A9