A0A651F6E4 · A0A651F6E4_9BACT

  • Protein
    tRNA-dihydrouridine(20/20a) synthase
  • Gene
    dusA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

FMN (UniProtKB | Rhea| CHEBI:58210 )

Features

Showing features for binding site, site, active site.

Type
IDPosition(s)Description
Binding site21-23FMN (UniProtKB | ChEBI)
Binding site74FMN (UniProtKB | ChEBI)
Site101Interacts with tRNA
Active site104Proton donor
Binding site143FMN (UniProtKB | ChEBI)
Binding site176FMN (UniProtKB | ChEBI)
Site188Interacts with tRNA; defines subfamily-specific binding signature
Site191Interacts with tRNA
Binding site216-218FMN (UniProtKB | ChEBI)
Binding site238-239FMN (UniProtKB | ChEBI)
Site305Interacts with tRNA; defines subfamily-specific binding signature
Site308Interacts with tRNA; defines subfamily-specific binding signature

GO annotations

AspectTerm
Cellular Componentmembrane
Molecular Functionflavin adenine dinucleotide binding
Molecular FunctionFMN binding
Molecular FunctiontRNA binding
Molecular FunctiontRNA-dihydrouridine20 synthase activity
Molecular FunctiontRNA-dihydrouridine20a synthase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA-dihydrouridine(20/20a) synthase
  • EC number
  • Alternative names
    • DusA-like U20-specific dihydrouridine synthase
      (U20-specific Dus
      )

Gene names

    • Name
      dusA
    • ORF names
      EA357_04850

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • DLM2.Bin89
  • Taxonomic lineage
    Bacteria > Bdellovibrionota > Bdellovibrionia > Bdellovibrionales > Pseudobdellovibrionaceae > Micavibrio

Accessions

  • Primary accession
    A0A651F6E4

Proteomes

Subcellular Location

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane32-54Helical

Keywords

  • Cellular component

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain19-316DUS-like FMN-binding

Sequence similarities

Belongs to the Dus family. DusA subfamily.
Belongs to the dus family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    349
  • Mass (Da)
    39,031
  • Last updated
    2020-04-22 v1
  • Checksum
    43C9A8AE293B6FDF
MTEFQHKPLPNGPDRRISVAPMMGWTDRHDRYFLRLIAPNILLYTVMLTTGALLHGDRRGFLRFDPAEHPVALQLGGSDPADLAKAAKYGEAEGYDEINLNCGCPSDRVQRGAFGACLMAKPRLVADCVAAMRDAVSVPVTVKTRIGIDDHDSYGFLCDFIGTISGKGGCDTFIVHARKAWLKGLNPKQNRTVPPLRRDIVRRIKQDFPQLTIITNGGLASEEDVTEELRHTDGVMIGREAYQNPYFLSSLERNVLGTPAHRLRSRRQVIEDLIPYMEREMRSGTALKDIARHILGLFQGLPGARNWRHHLSENACRPGAQISDITAALAHIHDIHDNPQEMPLHRRAE

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
REDF01000039
EMBL· GenBank· DDBJ
TVQ83756.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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