A0A651F6E4 · A0A651F6E4_9BACT
- ProteintRNA-dihydrouridine(20/20a) synthase
- GenedusA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids349 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs.
Catalytic activity
- 5,6-dihydrouridine20 in tRNA + NAD+ = uridine20 in tRNA + NADH + H+
- 5,6-dihydrouridine(20a) in tRNA + NAD+ = uridine(20a) in tRNA + NADH + H+
- 5,6-dihydrouridine(20a) in tRNA + NADP+ = uridine(20a) in tRNA + NADPH + H+
Cofactor
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 21-23 | FMN (UniProtKB | ChEBI) | |||
Binding site | 74 | FMN (UniProtKB | ChEBI) | |||
Site | 101 | Interacts with tRNA | |||
Active site | 104 | Proton donor | |||
Binding site | 143 | FMN (UniProtKB | ChEBI) | |||
Binding site | 176 | FMN (UniProtKB | ChEBI) | |||
Site | 188 | Interacts with tRNA; defines subfamily-specific binding signature | |||
Site | 191 | Interacts with tRNA | |||
Binding site | 216-218 | FMN (UniProtKB | ChEBI) | |||
Binding site | 238-239 | FMN (UniProtKB | ChEBI) | |||
Site | 305 | Interacts with tRNA; defines subfamily-specific binding signature | |||
Site | 308 | Interacts with tRNA; defines subfamily-specific binding signature | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | FMN binding | |
Molecular Function | tRNA binding | |
Molecular Function | tRNA-dihydrouridine20 synthase activity | |
Molecular Function | tRNA-dihydrouridine20a synthase activity |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-dihydrouridine(20/20a) synthase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bdellovibrionota > Bdellovibrionia > Bdellovibrionales > Pseudobdellovibrionaceae > Micavibrio
Accessions
- Primary accessionA0A651F6E4
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 32-54 | Helical | |||
Keywords
- Cellular component
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 19-316 | DUS-like FMN-binding | |||
Sequence similarities
Belongs to the Dus family. DusA subfamily.
Belongs to the dus family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length349
- Mass (Da)39,031
- Last updated2020-04-22 v1
- Checksum43C9A8AE293B6FDF
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
REDF01000039 EMBL· GenBank· DDBJ | TVQ83756.1 EMBL· GenBank· DDBJ | Genomic DNA |