A0A650CH82 · A0A650CH82_SULOH

  • Protein
    Carbamoyl phosphate synthase small chain
  • Gene
    carA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site53L-glutamine (UniProtKB | ChEBI)
Binding site234L-glutamine (UniProtKB | ChEBI)
Binding site236L-glutamine (UniProtKB | ChEBI)
Active site262Nucleophile
Binding site263L-glutamine (UniProtKB | ChEBI)
Binding site266L-glutamine (UniProtKB | ChEBI)
Binding site304L-glutamine (UniProtKB | ChEBI)
Binding site306L-glutamine (UniProtKB | ChEBI)
Binding site307L-glutamine (UniProtKB | ChEBI)
Active site347
Active site349

GO annotations

AspectTerm
Cellular Componentcarbamoyl-phosphate synthase complex
Molecular FunctionATP binding
Molecular Functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
Biological Process'de novo' pyrimidine nucleobase biosynthetic process
Biological Process'de novo' UMP biosynthetic process
Biological Processarginine biosynthetic process
Biological Processglutamine metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Carbamoyl phosphate synthase small chain
  • EC number
  • Alternative names
    • Carbamoyl phosphate synthetase glutamine chain

Gene names

    • Name
      carA
    • ORF names
      D1869_08445
      , HNQ62_000041

Organism names

  • Taxonomic identifier
  • Strains
    • TA-1
    • DSM 12421
  • Taxonomic lineage
    Archaea > Thermoproteota > Thermoprotei > Sulfolobales > Sulfolobaceae > Sulfurisphaera

Accessions

  • Primary accession
    A0A650CH82

Proteomes

Subcellular Location

Interaction

Subunit

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

Family & Domains

Features

Showing features for region, domain.

Type
IDPosition(s)Description
Region1-185CPSase
Domain9-146Carbamoyl-phosphate synthase small subunit N-terminal

Sequence similarities

Belongs to the CarA family.
Belongs to the CarB family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    372
  • Mass (Da)
    41,629
  • Last updated
    2020-04-22 v1
  • Checksum
    BADCC9BBB2BC4027
MTLYCKRGYKGYLYLEDGTLIEGCGFGAKGIRAGEVVFTTSMNGYPESLTDPSYRGQILVITHPLVGNYGVPEKQRVEGILTNFESEQIQVEGLVVAEETDPFKWNSSKSLHEWLLSEGVPGLSDVDTRSIVKKVRSRGVMMGVIASGYEIEDPKKFLEKKYDEIDFTQFTSPKAPIVHLGNTGETIVVVDCGVKHGILYQLHQRGFTIVRVPCKFNASKIMDYYPKGVVFGNGPGNPNLLQEVIENFKELTEYKIPILGICLGHQVATLAMGGKVNKMKFGHRAINKPVIDISSNKCYITTHNHGYGILSKEDLPPNSKLWFINPDDGTIEGWIHEKLPIITTQFHPEARPGPWDVTWVFDKFKKMVSRNA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JACHFY010000001
EMBL· GenBank· DDBJ
MBB5252323.1
EMBL· GenBank· DDBJ
Genomic DNA
CP045484
EMBL· GenBank· DDBJ
QGR17214.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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