A0A650CDH8 · A0A650CDH8_SULOH

Function

function

Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 3 Mg2+ cations per subunit. The strongest magnesium site (Mg1) is bound to the beta- and gamma-phosphates of ATP and four water molecules complete its coordination sphere.

Features

Showing features for site, binding site.

TypeIDPosition(s)Description
Site82Important for tRNA non-discrimination
Binding site167L-aspartate (UniProtKB | ChEBI)
Binding site210L-aspartate (UniProtKB | ChEBI)
Binding site210-212ATP (UniProtKB | ChEBI)
Binding site352ATP (UniProtKB | ChEBI)
Binding site352Mg2+ 2 (UniProtKB | ChEBI)
Binding site352Mg2+ 3 (UniProtKB | ChEBI)
Binding site355L-aspartate (UniProtKB | ChEBI)
Binding site355Mg2+ 2 (UniProtKB | ChEBI)
Binding site359L-aspartate (UniProtKB | ChEBI)
Binding site400-403ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionaspartate-tRNA ligase activity
Molecular Functionaspartate-tRNA(Asn) ligase activity
Molecular FunctionATP binding
Molecular Functionmagnesium ion binding
Molecular Functionnucleic acid binding
Biological Processaspartyl-tRNA aminoacylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Aspartate--tRNA(Asp/Asn) ligase
  • EC number
  • Alternative names
    • Aspartyl-tRNA synthetase
      (AspRS
      )
    • Non-discriminating aspartyl-tRNA synthetase
      (ND-AspRS
      )

Gene names

    • Name
      aspS
    • ORF names
      D1869_00530
      , HNQ62_000994

Organism names

  • Taxonomic identifier
  • Strains
    • TA-1
    • DSM 12421
  • Taxonomic lineage
    Archaea > Thermoproteota > Thermoprotei > Sulfolobales > Sulfolobaceae > Sulfurisphaera

Accessions

  • Primary accession
    A0A650CDH8

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain134-429Aminoacyl-transfer RNA synthetases class-II family profile
Region189-192Aspartate

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    429
  • Mass (Da)
    49,118
  • Last updated
    2020-04-22 v1
  • Checksum
    7CB61E38BD9F4CF6
MYRSHFIADVTPEYDGKEVIWAGWVHLLRDLGGKKFIILRDKTGLGQVVVDKNSSAFGISQELTQESVIQVRGIVKADKRAPRGIELHAEEITLLSKAKAPLPLDVSGKVKADIDTRLRERVLDLRRQEMQAVIKIQSLALKAFRETLYKEGFIEIFTPKIIASATEGGAQLFPVIYFGKEAFLAQSPQLYKELMAGVVERVFEVAPAWRAEESDTPFHLAEFISMDVEMAFADYNDVMQLLEKILHNIVKTIKEEGKEELKILNYEPPEVKIPIKRLKYTEAIEILRSKGYNIKFGDDIGTPELRILNEELKEDLYFIIDWPSDARPFYTKSKSENPELSESFDLIYKFLEIVSGSTRNHKREVLEETLKKKGLKPESFEFFLKWFDYGMPPHAGFGMGLARLMVMLTGIQSVKEIVPFPRDKKRLTP

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JACHFY010000003
EMBL· GenBank· DDBJ
MBB5253252.1
EMBL· GenBank· DDBJ
Genomic DNA
CP045484
EMBL· GenBank· DDBJ
QGR15842.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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