A0A641FYI1 · A0A641FYI1_ECOLX
- Protein3'-phosphate/5'-hydroxy nucleic acid ligase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids383 (go to sequence)
- Protein existencePredicted
- Annotation score2/5
Function
Catalytic activity
- a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP = a ribonucleotidyl-ribonucleotide-RNA + diphosphate + GMP
Cofactor
Note: Binds 2 manganese ions per subunit.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 78 | Mn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 140-144 | GMP (UniProtKB | ChEBI) | ||||
Sequence: NHFAE | ||||||
Binding site | 141 | Mn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 172 | Mn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 243 | Mn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 243-244 | GMP (UniProtKB | ChEBI) | ||||
Sequence: HN | ||||||
Binding site | 281 | GMP (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Active site | 298 | GMP-histidine intermediate | ||||
Sequence: H | ||||||
Binding site | 298-301 | GMP (UniProtKB | ChEBI) | ||||
Sequence: HGAG | ||||||
Binding site | 376 | GMP (UniProtKB | ChEBI) | ||||
Sequence: K |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | GTP binding | |
Molecular Function | metal ion binding | |
Molecular Function | RNA ligase (GTP) activity | |
Biological Process | RNA processing | |
Biological Process | RNA repair |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name3'-phosphate/5'-hydroxy nucleic acid ligase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionA0A641FYI1
Proteomes
Structure
Sequence
- Sequence statusComplete
- Length383
- Mass (Da)42,035
- Last updated2020-04-22 v1
- Checksum813494457FCB337F