A0A5Z1WQN2 · A0A5Z1WQN2_CAMJU
- ProteinInosine-5'-monophosphate dehydrogenase
- GeneguaB
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids485 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.
Catalytic activity
- IMP + NAD+ + H2O = XMP + NADH + H+
Cofactor
Activity regulation
Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.
Pathway
Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 244 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 244-246 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 294-296 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 296 | K+ (UniProtKB | ChEBI); ligand shared between two tetrameric partners; in other chain | |||
Binding site | 298 | K+ (UniProtKB | ChEBI); ligand shared between two tetrameric partners; in other chain | |||
Binding site | 299 | IMP (UniProtKB | ChEBI) | |||
Active site | 301 | Thioimidate intermediate | |||
Binding site | 301 | K+ (UniProtKB | ChEBI); ligand shared between two tetrameric partners; in other chain | |||
Binding site | 334-336 | IMP (UniProtKB | ChEBI) | |||
Binding site | 357-358 | IMP (UniProtKB | ChEBI) | |||
Binding site | 381-385 | IMP (UniProtKB | ChEBI) | |||
Active site | 397 | Proton acceptor | |||
Binding site | 411 | IMP (UniProtKB | ChEBI) | |||
Binding site | 465 | K+ (UniProtKB | ChEBI); ligand shared between two tetrameric partners | |||
Binding site | 466 | K+ (UniProtKB | ChEBI); ligand shared between two tetrameric partners | |||
Binding site | 467 | K+ (UniProtKB | ChEBI); ligand shared between two tetrameric partners | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | IMP dehydrogenase activity | |
Molecular Function | metal ion binding | |
Molecular Function | nucleotide binding | |
Biological Process | GMP biosynthetic process | |
Biological Process | GTP biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameInosine-5'-monophosphate dehydrogenase
- EC number
- Short namesIMP dehydrogenase ; IMPD ; IMPDH
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Campylobacterota > Epsilonproteobacteria > Campylobacterales > Campylobacteraceae > Campylobacter
Accessions
- Primary accessionA0A5Z1WQN2
Proteomes
Interaction
Subunit
Homotetramer.
Structure
Sequence
- Sequence statusComplete
- Length485
- Mass (Da)52,194
- Last updated2020-04-22 v1
- ChecksumB9D4BD368A2B20C2
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AACAAG010000001 EMBL· GenBank· DDBJ | EAJ7052998.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AACBBH010000002 EMBL· GenBank· DDBJ | EAJ8288713.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AACBZE010000001 EMBL· GenBank· DDBJ | EAJ8963728.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AACCII010000001 EMBL· GenBank· DDBJ | EAJ9718072.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AACKOK010000001 EMBL· GenBank· DDBJ | EAL0023837.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AACKPS010000007 EMBL· GenBank· DDBJ | EAL0078570.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AACPOI010000005 EMBL· GenBank· DDBJ | EAL5936304.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AACSEO010000001 EMBL· GenBank· DDBJ | EAL8964025.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AACTES010000001 EMBL· GenBank· DDBJ | EAM0223075.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAJBAM010000001 EMBL· GenBank· DDBJ | ECK2339877.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAJDCU010000001 EMBL· GenBank· DDBJ | ECK7581147.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAJDDU010000002 EMBL· GenBank· DDBJ | ECK7680289.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAJDKN010000002 EMBL· GenBank· DDBJ | ECK7960625.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAJERN010000001 EMBL· GenBank· DDBJ | ECL0461301.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKCAK010000001 EMBL· GenBank· DDBJ | ECQ6520850.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKFTH010000001 EMBL· GenBank· DDBJ | ECR3388453.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAMHGK010000015 EMBL· GenBank· DDBJ | EDH3326223.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AANOAG010000002 EMBL· GenBank· DDBJ | EDP7180372.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
MKBD01000001 EMBL· GenBank· DDBJ | OEY03961.1 EMBL· GenBank· DDBJ | Genomic DNA |