A0A5Q2V3U5 · A0A5Q2V3U5_9REOV

  • Protein
    Protein VP3
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Organism
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Has affinity for ssRNA but not for dsRNA. Capping activity is non-specific and caps RNAs that initiate with either a G or an A residue. Together with VP1 polymerase, forms a VP1-VP3 complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores do not.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

GO annotations

AspectTerm
Cellular Componentviral nucleocapsid
Molecular FunctionGTP binding
Molecular FunctionmRNA 5'-cap (guanine-N7-)-methyltransferase activity
Molecular FunctionmRNA guanylyltransferase activity
Molecular FunctionRNA binding
Biological Processvirus-mediated perturbation of host defense response

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Protein VP3

Including 2 domains:

  • Recommended name
    mRNA guanylyltransferase
  • EC number
  • Recommended name
    mRNA (guanine-N(7))-methyltransferase
  • EC number

Organism names

  • Taxonomic identifier
  • Organism
    Rotavirus C
  • Strain
    • RVC/Pig-wt/ESP/P1C/2017/GXP[4]
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Duplornaviricota > Resentoviricetes > Reovirales > Sedoreoviridae > Rotavirus

Accessions

  • Primary accession
    A0A5Q2V3U5

Subcellular Location

Virion
Note: Attached inside the inner capsid as a minor component. There are about 11 to 12 copies per virion.

Keywords

  • Cellular component

Interaction

Subunit

Interacts with VP1. Interacts with VP2.

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region433-559N7-methyltransferase activity
Region560-693GTase/RTPase activity

Sequence similarities

Belongs to the rotavirus VP3 family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    693
  • Mass (Da)
    81,611
  • Last updated
    2020-02-26 v1
  • Checksum
    77FB2E75027A5059
MRVLGLFERGNNLNFADTYVYTWNEQYSYHENAFLISNQVATTIIIYLSDTIINEVNEAFELLNSNGIPALIIKKDHVGIFTSSNFTYDWHHKIVYFHEYTYYKNNEFIVSDEFWLHTNMSELLPYKILYYERGMRKLYEGDEYTLYNTATDDDILYKYIYEKDMIMDGDDYTELYDDKNFRNFVHFMRLLRMRFAVPFDQLSNRITRSRVFFKSKVHVGLRNESIPQALDNIHSQWINYSANGITISELKGSGSYSEKRISEFDIGQFKNYMNFLTLMFYIKNMRKRPSCTIIGAAPGYWIPSMKKYFTIITYDDKKVDSTEHHNRYFSEEDIVNVRTNGVYIDVRSDFNTNDWKKRRRLVEEETKRWLDISYKLLEGRYVEAILLKMTAMDIEIPDGYFIHFPTTYRKSEYYLLIDKQIIKKQKVKVTKSLMYNAINTIYSDNVFVSGKYTLRGKTEGVVALYYLSNTINPKDKVIQYANSFSGTCMTVRLNNTYEVNKIIDFKTNADYTFLPSDFACSPKTVLTSYRGYAGVFGYAITKDLKSDGNNHIYIIPNARDESNFDTFASHLGLSRYSHSKRFSESATTMSGYLFRDMVSGKEDMEDTDKANCASGHVFNAIAHYRFDYTYDIVGWLRLHKTKHFKVKSDIYEEHTDDEVRNAIEAAYTYYLLDGDKVGKEYAKKIMEIWEAQV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MK953050
EMBL· GenBank· DDBJ
QGH58855.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

Disclaimer

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