A0A5Q2F3P4 · A0A5Q2F3P4_9CAUD

Function

function

The terminase large subunit acts as an ATP driven molecular motor necessary for viral DNA translocation into empty capsids and as an endonuclease that cuts the viral genome at a unique and precise dsDNA sequence to initiate and to end a packaging reaction. The terminase lies at a unique vertex of the procapsid and is composed of two subunits, a small terminase subunit involved in viral DNA recognition (packaging sequence), and a large terminase subunit possessing endonucleolytic and ATPase activities. Both terminase subunits heterooligomerize and are docked on the portal protein to form the packaging machine. The terminase large subunit exhibits endonuclease activity and cleaves the viral genome concatemer. Once the DNA is packaged, the terminase detaches from the portal and gets replaced by the tail to finish maturation of the virion.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site.

158550100150200250300350400450500550
TypeIDPosition(s)Description
Binding site365Mg2+ 1 (UniProtKB | ChEBI); catalytic; for nuclease activity
Binding site365Mg2+ 2 (UniProtKB | ChEBI); catalytic; for nuclease activity
Binding site420Mg2+ 2 (UniProtKB | ChEBI); catalytic; for nuclease activity
Binding site518Mg2+ 1 (UniProtKB | ChEBI); catalytic; for nuclease activity

GO annotations

AspectTerm
Cellular Componentviral terminase, large subunit
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular Functionendonuclease activity
Molecular Functionmetal ion binding
Biological Processchromosome organization
Biological Processviral DNA genome packaging

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Terminase, large subunit
  • Alternative names
    • DNA-packaging protein

Including 2 domains:

  • Recommended name
    ATPase
  • EC number
  • Recommended name
    Endonuclease
  • EC number

Gene names

    • ORF names
      vBKp168_5

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Viruses > Duplodnaviria > Heunggongvirae > Uroviricota > Caudoviricetes > Autographiviridae > Studiervirinae > Przondovirus > Przondovirus Kp1

Accessions

  • Primary accession
    A0A5Q2F3P4

Proteomes

Subcellular Location

Interaction

Subunit

Homopentamer. Interacts with the terminase small subunit; the active complex is probably heterooligomeric. Interacts with the portal protein.

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif59-66Walker A motif
Motif157-162Walker B motif

Domain

The ATPase region is in the N-terminus, whereas the nuclease region is in the central part. The C-terminus is involved in prohead binding.

Sequence similarities

Belongs to the Teseptimavirus large terminase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    585
  • Mass (Da)
    66,184
  • Last updated
    2020-02-26 v1
  • Checksum
    491B3115CEB180B2
MSNSQQAKNALIIAQLKGDFVAFLFVLWKALNLPEPTKCQIDMAKCLADPKNKKFILQAFRGIGKSFITCAFVVWTLWRDPQLKILIVSASKERADANSIFIKNIIDLLPFLSELKPRPGQRDSVISFDVGPAKPDHSPSVKSVGITGQLTGSRADIIIADDVEIPGNSATQGAREKLWTLVQEFAALLKPLPTSRVIYLGTPQTEMTLYKELEDNRGYSTIIWPAQYPRSKEEDLYYGDRLAPMLRSEYDEDKEGLSSQPTDPVRFDSMDLQEREVEYGKAGYTLQFMLNPNLSDAEKYPLRLRDAIVCGLQADKAPMHYQWLPNRQNLNEELPNVGMKGDEIYSFHAASSNTGAYQGKILVIDPSGRGKDETGWCVLYTLNGYIYLMDAGGTRGYEEKSLEFLAKKAKQWQVQTVVFESNFGDGMFGNVFQPVLLKHHPAQLEEIRARGMKEVRICDTLEPVLASHRLVIRDEVIRQDYQTARDADGKHALKYSLFYQMTRMSREKGAVAHDDRLDALALGVEFLRSTMQQDAVKIEAEVLQEFLEHHMEKPLSNISQFRATSSNGVDIRWEDDGDDTMFIAW

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MN585130
EMBL· GenBank· DDBJ
QGF21389.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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