A0A5P1RHA8 · A0A5P1RHA8_9GAMM
- ProteinCarbamoyltransferase HypF
- GenehypF
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids772 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Involved in the maturation of [NiFe] hydrogenases. Along with HypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases. HypF functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carboxamido moiety in an ATP-dependent reaction to the thiolate of the C-terminal cysteine of HypE yielding a protein-S-carboxamide.
Catalytic activity
- C-terminal L-cysteinyl-[HypE protein] + carbamoyl phosphate + ATP + H2O = C-terminal S-carboxamide-L-cysteinyl-[HypE protein] + AMP + phosphate + diphosphate + H+
Pathway
Protein modification; [NiFe] hydrogenase maturation.
Features
Showing features for active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 23 | ||||
Active site | 41 | ||||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | acylphosphatase activity | |
Molecular Function | carboxyl- or carbamoyltransferase activity | |
Molecular Function | double-stranded RNA binding | |
Molecular Function | ligase activity | |
Molecular Function | zinc ion binding | |
Biological Process | protein carbamoylation | |
Biological Process | protein maturation |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCarbamoyltransferase HypF
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Oceanospirillales > Oceanospirillaceae > Neptunomonas
Accessions
- Primary accessionA0A5P1RHA8
Proteomes
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 8-95 | Acylphosphatase-like | |||
Domain | 205-396 | YrdC-like | |||
Sequence similarities
Belongs to the carbamoyltransferase HypF family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length772
- Mass (Da)86,034
- Last updated2020-04-22 v1
- MD5 Checksum73AE19FB99F82793E64E08F23EF3A3E4
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP043869 EMBL· GenBank· DDBJ | QEQ98406.1 EMBL· GenBank· DDBJ | Genomic DNA |