A0A5N8YBG6 · A0A5N8YBG6_9CHLR

Function

function

Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 1 Mg2+ or Mn2+ ion per subunit.
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site76-89NAD+ (UniProtKB | ChEBI)
Binding site96substrate
Binding site106substrate
Binding site134substrate
Site141Important for catalysis
Site191Important for catalysis
Binding site223Mg2+ (UniProtKB | ChEBI)
Binding site223substrate
Binding site247Mg2+ (UniProtKB | ChEBI)
Binding site251Mg2+ (UniProtKB | ChEBI)
Binding site291-303NAD+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function3-isopropylmalate dehydrogenase activity
Molecular Functionmagnesium ion binding
Molecular FunctionNAD binding
Biological ProcessL-leucine biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    3-isopropylmalate dehydrogenase
  • EC number
  • Alternative names
    • 3-IPM-DH
    • Beta-IPM dehydrogenase
      (IMDH
      )

Gene names

    • Name
      leuB
    • ORF names
      FIM08_00515

Organism names

Accessions

  • Primary accession
    A0A5N8YBG6

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain4-363Isopropylmalate dehydrogenase-like

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    369
  • Mass (Da)
    40,089
  • Last updated
    2020-04-22 v1
  • Checksum
    C1E4489837A13B1F
MQFNIALLPGDGVGPEVVAEGVRALKRVEELTDATFNLEYGQVGGIAIDEKGTPLPNEALELARRSDAILFGAVGGPKWDNPLAPHRPEDAILGLRKALGFFANLRPVKVIPALLNSSSVKPSVLEGVDMLVVRELTGGLYFARPKKRWQTSRGRRGVDTLRYTEAEIERVLRVGFELARRRRKKLTSVDKANVLESSRLWREIAIELSGQYPDVELEHMLVDSCAMALVRTPIRFDVLVAENLFGDILTDEAAILAGSLGMLPSSSLAGIPREDTGGHRRTLGLYEPIHGSAPDIAGQGKANPIATILSVALMLRYSLGHPNAASALEAAVESVLNQGYRTQDIAEEGSRDVLTTAEMGQRIVSDMRL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
VEXC01000002
EMBL· GenBank· DDBJ
MQF48378.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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