A0A5N4E451 · A0A5N4E451_CAMDR
- ProteinATP-dependent DNA helicase PIF1
- GenePIF1
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids645 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Possesses an intrinsic strand annealing activity.
Catalytic activity
- ATP + H2O = ADP + phosphate + H+
Cofactor
Features
Showing features for binding site, dna binding.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 228-235 | ATP (UniProtKB | ChEBI) | |||
DNA binding | 581-600 | ||||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrion | |
Cellular Component | nucleus | |
Molecular Function | 5'-3' DNA helicase activity | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | G-quadruplex DNA binding | |
Molecular Function | telomerase inhibitor activity | |
Biological Process | DNA recombination | |
Biological Process | DNA repair | |
Biological Process | mitochondrial genome maintenance | |
Biological Process | negative regulation of telomere maintenance via telomerase | |
Biological Process | telomere maintenance |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent DNA helicase PIF1
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Tylopoda > Camelidae > Camelus
Accessions
- Primary accessionA0A5N4E451
Proteomes
Subcellular Location
Interaction
Subunit
Monomer. Interacts with telomerase.
Structure
Sequence
- Sequence statusComplete
- Length645
- Mass (Da)70,123
- Last updated2020-02-26 v1
- Checksum440644B72F11B09A
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A5N4E486 | A0A5N4E486_CAMDR | PIF1 | 641 | ||
A0A5N4E4H9 | A0A5N4E4H9_CAMDR | PIF1 | 648 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JWIN03000006 EMBL· GenBank· DDBJ | KAB1278248.1 EMBL· GenBank· DDBJ | Genomic DNA |