A0A5N4E451 · A0A5N4E451_CAMDR

Function

function

DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Possesses an intrinsic strand annealing activity.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site, dna binding.

Type
IDPosition(s)Description
Binding site228-235ATP (UniProtKB | ChEBI)
DNA binding581-600

GO annotations

AspectTerm
Cellular Componentmitochondrion
Cellular Componentnucleus
Molecular Function5'-3' DNA helicase activity
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular FunctionG-quadruplex DNA binding
Molecular Functiontelomerase inhibitor activity
Biological ProcessDNA recombination
Biological ProcessDNA repair
Biological Processmitochondrial genome maintenance
Biological Processnegative regulation of telomere maintenance via telomerase
Biological Processtelomere maintenance

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    ATP-dependent DNA helicase PIF1
  • EC number
  • Alternative names
    • DNA repair and recombination helicase PIF1

Gene names

    • Name
      PIF1
    • ORF names
      Cadr_000005159

Organism names

  • Taxonomic identifier
  • Strain
    • Drom800
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Tylopoda > Camelidae > Camelus

Accessions

  • Primary accession
    A0A5N4E451

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Monomer. Interacts with telomerase.

Family & Domains

Features

Showing features for region, domain.

Type
IDPosition(s)Description
Region126-202Disordered
Domain465-508DNA helicase Pif1-like 2B

Sequence similarities

Belongs to the helicase family. PIF1 subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    645
  • Mass (Da)
    70,123
  • Last updated
    2020-02-26 v1
  • Checksum
    440644B72F11B09A
MLSGTQAAAEECEDAELQCRVAVEELSPGGQPRRRQALRSAELSLGRNERRELMLRLQAPGPAGRPRCFPLRAARLFTRFAAEGRSTLRLPADSAPRAGAVQLLLSDCPPDRLRRFLRTLRLKLAADPGPGPASARTQLLGPRPRDFVTISPLQPEELRRAAATRVTDTTPVKRPTEPRSETKPSTESPRWPLPVKKLSLPSTKPQLSKEQAAVLRVVLKGQSIFFTGSAGTGKSYLLKRILGSLPPTGTVATASTGVAACHIGGTTLHAFAVLLAGIGSGQAPLAQCVALAQRPGVRQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQNKPFGGIQLIICGDFLQLPPVTKGSQPPQFCFQAKSWRRCVPVILELTEVWRQADKTFISLLQAVRLGRCSDEVTRQLRATAAHKVGRDGIVATRLCTHQDDVALTNEKRLQELPGEIHSFEAMDSDPEQARTLDAQCPVSQLLQLKLGAQVMLVKNLAVSRGLVNGARGVVVGFEAEGRGLPQVRFLCGVTEVIRADRWTVQATGGQLLSRQQLPLQLAWAISIHKSQGMSLDCVEISLSRVFASGQAYVALSRARSLQGLRVLDFDPMVVRCDPRVLSFYATLRQARGLSLGSPNDSEAASDQENVDPNL

Computationally mapped potential isoform sequences

There are 2 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A5N4E486A0A5N4E486_CAMDRPIF1641
A0A5N4E4H9A0A5N4E4H9_CAMDRPIF1648

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JWIN03000006
EMBL· GenBank· DDBJ
KAB1278248.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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