A0A5K1JVJ2 · A0A5K1JVJ2_9APHY

  • Protein
    NADPH-dependent diflavin oxidoreductase 1
  • Gene
    Q0P7K6
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

NADPH-dependent reductase which is a central component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Transfers electrons from NADPH via its FAD and FMN prosthetic groups to the [2Fe-2S] cluster of DRE2, another key component of the CIA machinery. In turn, this reduced cluster provides electrons for assembly of cytosolic iron-sulfur cluster proteins. Positively controls H2O2-induced cell death.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
FAD (UniProtKB | Rhea| CHEBI:57692 )

FMN (UniProtKB | Rhea| CHEBI:58210 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site35-40FMN (UniProtKB | ChEBI)
Binding site82-85FMN (UniProtKB | ChEBI)
Binding site155FMN (UniProtKB | ChEBI)
Binding site395-398FAD (UniProtKB | ChEBI)
Binding site429-432FAD (UniProtKB | ChEBI)
Binding site470NADP+ (UniProtKB | ChEBI)
Binding site530-531NADP+ (UniProtKB | ChEBI)
Binding site539-543NADP+ (UniProtKB | ChEBI)
Binding site614FAD (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentmitochondrion
Molecular Functionflavin adenine dinucleotide binding
Molecular FunctionFMN binding
Molecular Functionhydrolase activity
Molecular FunctionNADP binding
Molecular FunctionNADPH-hemoprotein reductase activity
Biological Processiron-sulfur cluster assembly

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NADPH-dependent diflavin oxidoreductase 1
  • EC number
  • Alternative names
    • NADPH-dependent FMN and FAD-containing oxidoreductase

Gene names

    • Name
      Q0P7K6
    • Synonyms
      TAH18

Organism names

  • Taxonomic identifier
  • Organism
  • Taxonomic lineage
    Eukaryota > Fungi > Dikarya > Basidiomycota > Agaricomycotina > Agaricomycetes > Polyporales > Polyporaceae > Ganoderma

Accessions

  • Primary accession
    A0A5K1JVJ2

Subcellular Location

Cytoplasm
Mitochondrion
Note: Relocalizes to mitochondria after H2O2 exposure.

Keywords

Interaction

Subunit

Interacts with DRE2; as part of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery.

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-25Disordered
Domain29-173Flavodoxin-like
Domain220-456FAD-binding FR-type

Sequence similarities

Belongs to the NADPH-dependent diflavin oxidoreductase NDOR1 family.
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
In the N-terminal section; belongs to the flavodoxin family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    615
  • Mass (Da)
    68,717
  • Last updated
    2019-12-11 v1
  • Checksum
    230A9BA598876808
MSSPPPSPPSSSRCSTPSDGADPHDERTLTILYATETGTAQDVADRLARVCRCLHIHARVHSMDVYSPAELINEHLVVFVVSTTGSGREPRAMTPLWQMLLRADLPEDLFEDLHFAVFGLGDTAYEKFCWPAKLLSRRLEALGALELCARGEGDEQHHLGIEGALGPWIENLSTVLLQVYPLPHGLQRESFDSLPPPRVAIADADKDAQITRKDPLVADREYHTATVVVNRRLTAEDWYQDVRHFEFELPEDVEYDPGDVAVIHPEAMPQDVEAFLSCIGYADTADDPIEIRHALLDQHLPDHIPTITTMREIFTRYVDINAVPRRSFFALLKHFAQDDMEREKLEEFLTEEGADDLYEYCQKPHRRIHEVLSEFRSVHIPRAYVFDLFPPLRPRQFSIASSARADPRRIHLCVAIVAYRTMLKVPRRGVCTTWLLARAPGDRIQIGVQKGFIALPPDPATPLICVGPGTGVAPMRAVIQERLSVDAGVAGKDTLALYFGCRSEAKDHHYGAEWRALAAEGKLAYRVAFSRDGPEGKARTYVQDLIRADAPRIWELVGARGACVYISGSSNKMPAAVRAAIAHAVQTQGGRTEEGAKEYVAQMEREGRLIEECWS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LR725298
EMBL· GenBank· DDBJ
VWO96020.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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