A0A5J6AAT7 · A0A5J6AAT7_RUBV

Function

function

Probable principal replicase for the negative-strand DNA, which replicates the 40S + genomic RNA into - antigenomic RNA. It cannot replicate the - into + until cleaved into p150 and p90 mature proteins.
Protease that cleaves the precursor polyprotein into two mature products. Together with RNA-directed RNA polymerase p90, replicates the 40S genomic and antigenomic RNA by recognizing replications specific signals. The heterodimer P150/p90 is probably the principal replicase for positive-strand genomic RNA and the 24S subgenomic RNA, which codes for structural proteins. Responsible for the mRNA-capping of the viral mRNAs. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. Forms fibers late in the infection that may be involved in cell-to-cell spread of the virus RNA in the absence of virus particle formation.
Together with protease/methyltransferase p150, replicates the 40S genomic and antigenomic RNA by recognizing replications specific signals. The heterodimer P150/p90 is probably the principal replicase for positive-strand genomic RNA and the 24S subgenomic RNA, which codes for structural proteins. A helicase activity is probably also present.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Zn2+ is necessary for the protease activity. The protease can also function efficiently with Cd2+ and Co2+.

Features

Showing features for active site, binding site, site.

Type
IDPosition(s)Description
Active site1152For cysteine protease activity
Binding site1175Zn2+ (UniProtKB | ChEBI)
Binding site1178Zn2+ (UniProtKB | ChEBI)
Binding site1227Zn2+ (UniProtKB | ChEBI)
Active site1273For cysteine protease activity
Binding site1273Zn2+ (UniProtKB | ChEBI)
Site1301-1302Cleavage; autocatalytic

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componenthost cell cytoplasm
Cellular Componenthost cell membrane
Cellular Componentmembrane
Molecular FunctionATP binding
Molecular Functioncysteine-type endopeptidase activity
Molecular Functionmetal ion binding
Molecular FunctionmRNA methyltransferase activity
Molecular Functionribonucleoside triphosphate phosphatase activity
Molecular FunctionRNA binding
Molecular FunctionRNA helicase activity
Molecular FunctionRNA-dependent RNA polymerase activity
Biological ProcessDNA-templated transcription
Biological ProcessmRNA modification
Biological Processproteolysis
Biological ProcessRNA processing
Biological Processviral RNA genome replication

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Non-structural polyprotein p200

Organism names

  • Taxonomic identifier
  • Strain
    • RVs/Louisiana.USA/27.17/2[1a]
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Kitrinoviricota > Alsuviricetes > Hepelivirales > Matonaviridae > Rubivirus > Rubivirus rubellae

Accessions

  • Primary accession
    A0A5J6AAT7

Subcellular Location

Keywords

PTM/Processing

Post-translational modification

Non-structural polyprotein p200: Specific enzymatic cleavage by its own cysteine protease yield mature proteins p150 and p90.

Interaction

Subunit

Interacts with RNA-directed RNA polymerase p90. Interacts with host CALM1; this interaction is necessary for the protease activity and viral infectivity. Interacts with host C1QBP. Interacts with the capsid protein.
Interacts with human RB1/retinoblastoma protein. Interacts with protease/methyltransferase p150.

Family & Domains

Features

Showing features for domain, region, compositional bias.

Type
IDPosition(s)Description
Domain57-247Alphavirus-like MT
Region458-478Disordered
Region711-811Disordered
Compositional bias747-772Pro residues
Domain806-985Macro
Region990-1029Disordered
Domain1000-1301Peptidase C27
Region1152-1183Interaction with host CALM1
Region1193-1228EF-hand-like
Domain1320-1609+RNA virus helicase C-terminal
Domain1870-1981RdRp catalytic

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    2,116
  • Mass (Da)
    230,427
  • Last updated
    2019-12-11 v1
  • Checksum
    4FDB131BC52D7209
MERLLDEVLAPGGPYNLTVGSWVRDHVRSIVEGAWEVRDVVSAAQKRAIVAVIPRPVFTQMQVSDHPALHAISRYTRRHWIEWGPKEALHVLIDPSPGLLREXARVERHWVALCLHRTARKLATALAETASEAWHADYVCALRGAPSGPFYVHPEDVPHGGRAVADRCLLYYTPMQMCELMRTIDATLLVAVDLWPVALAAHVGDDWDDLGIAWHLDHDGGCPADCRGAGAGPTPGYTRPCTTRIYQVLPDTAHPGRLYRCGPRLWTRDCAVAELSWEVAQHCGHQARVRAVRCTLPIRHVRSLQPSARVRLPDLVHLAEVGRWRWFSLPRPVFQRMLSYCKTLSPDAYYSERVFKFKNALSHSITLAGNVLQEGWKGTCAEEDALCAYVAFRAWQSNARLAGIMKSAKRCAADSLSVAGWLDTIWGAIKRFFGSVPLAERMEEWEQDAAVAAFDRGPLEDGGRHLDTVQPPKSPPRPEIAATWIVHAASADRHCACAPRCDVPRERPSAPAGPPDDEALIPPWLFAERRALRCREWDFEVLRARADTAAVPAPLAPRPARYPTVLYRHPAHHGPWLTLDEPGEADAALVLCDPLGQPLRGPERHFAAGAHMCAQARGLQAFVRVVPPPERPWADGGARAWAKFFRGCAWAQRLLGEPAVMHLPYTDGDVPQLIALALRTLAQQGAALALSVRDLPGGAAFDANAVTAAVRSGPGQSAATSSPPGDPPPPRRARRLQRHSDARGTPPPAPVRDPPPPAPSPPAPPRAGDPVPPTSAGPADRARDAELEVAYEPSGPPSSTKADPDSDIVESYARAAGPVHLRVRDIMDPPPGCKVVVNAANEGLLAGSGVCGAIFANATAALAADCRRLAPCPTGEAVATPGHGCGYTYIIHAVAPRRPRDPAALEEGEALLERAYRSIVALAAARRWAHVACPLLGAGVYGWSAAESLRAALAATRTEPAERVSLHICHPDRATLTHASVLVGAGLAARRVSPPPTEPLASCPAGDPGRPAQRSASPPATPLGDATAPEPRGCQGCELCRYTRVTNDRAYVNLWLERDRGATSWAMRIPEVVVYGPEHLATHFPLNHYSVLKPAEVRPPRGMCGSDMWRCRGWQGVPQVRCTPSNAHAALCRTGVPPRVSTRGGELDPNTCWLRAAANVAQAARACGAYTSAGCPRCAYGRALSEARTHEDFAALSQRWSASHADASSDGTGDPLDPLMETVGCACSRVWVGSEHEAPPDHLLVSLHRAPNGPWGVVLEVRARPEGGNPTGHFVCAVGGGPRRVSDRPHLWLAVPLSRGGGTCAATDEGLAQAYYDDLEVRRLGDDAMARAALASVQRPRKGPYNIRVWNMAAGAGKTTRILAAFTREDLYVCPTNALVHEIQAKLRARDIDVKNAATYERALTKPLAAYRRIYIDEAFTLGGEYCAFVASQTTAEVICVGDRDQCGPHYANNCRTPVPDRWPTERSRHTWRFPDCWAARLRAGLDYDIEGERTGTFACNLWDGRQVDLHLAFSRETVRRLHEAGIRAYTVREAQGMSVSTACIHVGRDGTDVALALTRDLAIVSLTRASDALYLHELEDGSLRAAGLSAFCDAGALAELKEVPAGIDRVVAVEQAPPPLPPADGIPEAQDVPPFCPRTLEELVFGRAGHPHYADLNRVTEGEREVRYMRISRHLLNKNHTEMPGTERVLSAVCAVRRYRAGEDGSTLRTAVARQHPRPFRQIPPPRVTAGVAQEWRMTYLRERIDLTDVYTQMGVAARELADRYARRYPEIFAGMCTAQSLSVPAFLKATLKCVDAALGPRDTEDCHAAQGKAGLEIRAWAKEWVQVMSPHFRAIQKIIMRALRPQFLVAAGHTEPEVDAWWQAHYTTNAIEVDFTEFDMNQTLATRDVELEISAALLGLPCAEDYRALRAGSYCTLRELGSTETGCERTSGEPATLLHNTTVAMCMAMRMVPKGVRWAGIFQGDDMVIFLPEGARSAALKWTPAEVGLFGFHIPVKHVSTPTPSFCGHVGTAAGLFHDVMHQAIKVLCRRFDPDVLEEQQVALLDRLRGVYAALPDTVAANAAYYDYSAERVLAIVRELTAYARGRGLDHPATIGALEEIQTPYARANLHDAD

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias747-772Pro residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MK780809
EMBL· GenBank· DDBJ
QBZ68617.1
EMBL· GenBank· DDBJ
Genomic RNA

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