A0A5I6L3V5 · A0A5I6L3V5_SALET
- Protein5-keto-4-deoxy-D-glucarate aldolase
- GenegarL
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids256 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the reversible retro-aldol cleavage of both 5-keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde.
Catalytic activity
- 2-dehydro-3-deoxy-D-glucarate = 2-hydroxy-3-oxopropanoate + pyruvate
- 5-dehydro-4-deoxy-D-glucarate = 2-hydroxy-3-oxopropanoate + pyruvate
- D-glyceraldehyde + pyruvate = 2-dehydro-3-deoxy-L-galactonate
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Pathway
Carbohydrate acid metabolism; galactarate degradation; D-glycerate from galactarate: step 2/3.
Features
Showing features for active site, site, binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 50 | Proton acceptor | |||
Site | 75 | Transition state stabilizer | |||
Site | 89 | Increases basicity of active site His | |||
Binding site | 151 | substrate | |||
Binding site | 153 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 178 | substrate | |||
Binding site | 179 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 179 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 2-dehydro-3-deoxyglucarate aldolase activity | |
Molecular Function | magnesium ion binding | |
Biological Process | D-glucarate catabolic process | |
Biological Process | galactarate catabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name5-keto-4-deoxy-D-glucarate aldolase
- EC number
- Short namesKDGluc aldolase ; KDGlucA
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Salmonella
Accessions
- Primary accessionA0A5I6L3V5
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Subunit
Homohexamer; trimer of dimers.
Structure
Sequence
- Sequence statusComplete
- Length256
- Mass (Da)27,321
- Last updated2019-12-11 v1
- Checksum41EFEBE9EE3A2AFA
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AAHUCI010000014 EMBL· GenBank· DDBJ | ECA3602891.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAAQUJ010000008 EMBL· GenBank· DDBJ | HAE0919297.1 EMBL· GenBank· DDBJ | Genomic DNA |