A0A5G2QNM9 · A0A5G2QNM9_PIG
- ProteinAcyl-protein thioesterase 1
- GeneLYPLA1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids303 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Acts as an acyl-protein thioesterase. Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Acts as a palmitoyl thioesterase that catalyzes depalmitoylation of proteins, such as ADRB2, KCNMA1 and SQSTM1. Acts as a negative regulator of autophagy by mediating palmitoylation of SQSTM1, decreasing affinity between SQSTM1 and ATG8 proteins and recruitment of ubiquitinated cargo proteins to autophagosomes. Acts as a lysophospholipase and hydrolyzes lysophosphatidylcholine (lyso-PC). Also hydrolyzes lysophosphatidylethanolamine (lyso-PE), lysophosphatidylinositol (lyso-PI) and lysophosphatidylserine (lyso-PS). Has much higher thioesterase activity than lysophospholipase activity. Contributes to the production of lysophosphatidic acid (LPA) during blood coagulation by recognizing and cleaving plasma phospholipids to generate lysophospholipids which in turn act as substrates for ENPP2 to produce LPA.
Catalytic activity
- 1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + hexadecanoate + H+This reaction proceeds in the forward direction.
- a 1-(9Z-octadecenoyl)-2-acyl-sn-glycero-3-phosphocholine + H2O = a 2-acyl-sn-glycero-3-phosphocholine + (9Z)-octadecenoate + H+This reaction proceeds in the forward direction.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | cytosol | |
Cellular Component | endoplasmic reticulum | |
Cellular Component | nuclear membrane | |
Cellular Component | nucleoplasm | |
Cellular Component | plasma membrane | |
Molecular Function | carboxylic ester hydrolase activity | |
Molecular Function | lysophospholipase activity | |
Molecular Function | palmitoyl-(protein) hydrolase activity | |
Biological Process | fatty acid transport | |
Biological Process | negative regulation of aggrephagy | |
Biological Process | negative regulation of Golgi to plasma membrane protein transport | |
Biological Process | protein depalmitoylation |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAcyl-protein thioesterase 1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Suina > Suidae > Sus
Accessions
- Primary accessionA0A5G2QNM9
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
PTM/Processing
Keywords
- PTM
Expression
Gene expression databases
Interaction
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-61 | Disordered | ||||
Sequence: MRARGPRGQPGPPGAGWPDIVTRAAGGADGEARARGPGGRRGGGAHAPERAPVRGSDSPFA | ||||||
Domain | 108-298 | Phospholipase/carboxylesterase/thioesterase | ||||
Sequence: HGWAEAFAGIRSAHIKYICPHAPVMPVTLNMNMAMPSWFNIIGLSPDSLEDETGIKQAAENVKSLIEQEVKNGIPSNRIILGGFSQGGALSLYTALTTHQKLAGVTALSCWLPLRSSFPECPISGVNRDIPILQCHGVLDPLVPLMFGSLTVERLKTLVNPANVTFKTYEGMMHSSCQQEMLDVKQFIEKL |
Sequence similarities
Belongs to the AB hydrolase superfamily. AB hydrolase 2 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length303
- Mass (Da)32,408
- Last updated2019-12-11 v1
- Checksum33CDFBCD220286E5
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
F1RSG9 | F1RSG9_PIG | LYPLA1 | 230 | ||
A0A5G2RCG0 | A0A5G2RCG0_PIG | LYPLA1 | 293 | ||
A0A287A534 | A0A287A534_PIG | LYPLA1 | 196 |
Keywords
- Technical term